Quick Spin Columns for radiolabeled DNA purification

半岛bd体育手机客户端 说明

一般描述

Quick Spin columns contain gel filtration matrices which allow large molecules (e.g., DNA or RNA) to pass through quickly while retaining small molecules (e.g., nucleotides). The Quick Spin format improves the molecular sieving concept by using centrifugation to separate DNA or RNA rapidly and cleanly from small contaminants.
Quick Spin columns are produced with material that is shown to have a high recovery of radiolabeled DNA ( ≥80%).
All tedious, time-consuming steps involved in preparing columns have already been performed. The columns are pre-packed and quality tested to ensure

• maximal retention of unincorporated nucleotides (≥95%), and
• absence of DNase contamination according to the current Quality Control procedures.

Contents
Pre-packed, pre-swollen, pre-spun columns.
Suspension of Sephadex G-25 in STE buffer (10 mM Tris-HCl, pH 7.5, 1 mM EDTA, 100 mM NaCl).

特异性

Exclusion limit: 10-12 bp

应用

Ready-to-use disposable column units designed to quickly and efficiently remove unincorporated precursors from DNA labeled by nick translation, end-labeling, polymerization reactions, and other labeling techniques.
Quick Spin columns are designed for use in low-speed, swinging-bucket centrifuges.

分析说明

Sample size: up to 50 μl
Time required: 10 minuntes

其他说明

仅用于生命科学研究。不可用于诊断。

半岛bd体育手机客户端 性质

质量水平	100
包装	pkg of 20 columns (11273922001) pkg of 50 columns (11273949001)
manufacturer/tradename	Roche
运输	wet ice
储存温度	2-8℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash