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Anti-GRP94 (C-terminal) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
G4420
规格纯度
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
价格
4731.57 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-GRP94 (C-terminal) antibody produced in rabbit
Anti-Endoplasmin precursor
Anti-Tumor Rejection Antigen 1
Anti-Glucose Regulated Protein 94
Anti-gp96
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

免疫原

synthetic peptide corresponding to amino acids 733-750 located near the C-terminus of human GRP94. This sequence is identical in mouse, rat, dog, porcine, and bovine GRP94 and highly conserved (1 amino acid substitutuion) in chicken.

应用

Anti-GRP94 (C-terminal) antibody produced in rabbit is suitable for immunoblotting at a working concentration of 0.5-1μg/mL using whole cell extracts of the human epitheloid carcinoma HeLa cells, Madin Darby canine kidney (MDCK) cells, and mouse fibroblast NIH3T3 cells. It is also suitable for immunoprecipitation of GRP94 protein from a MDCK whole cell lysate at 10-20μg concentration.
Anti-GRP94 (C-terminal) antibody produced in rabbit has been used in immunocytochemistry.

生化/生理作用

The GRP94 (glucose-regulated protein 94) is a 94 kDa Ca2+- binding glycoprotein that belongs to a subfamily of the heat shock proteins Hsp90. It is a chaperone protein constitutively localized to the endoplasmic reticulum (ER) of mammalian cells.
GRP94 (glucose-regulated protein 94) is involved in protein folding, sorting and secretion, and binding of immunogenic peptides. GRP94 has a role in peptide antigen presentation to major histocompatibility complex class I molecules of antigen presenting cells (APCs). It binds specifically to CD91 (α2-macroglobulin receptor), and possibly to the Toll-like receptors (TLRs) on the surface of APCs. Overexpression of GRP94 has been associated with cellular transformation and tumorigenesis.
GRPs are unresponsive to heat stress and are induced by stress related to glucose starvation or defects in glycoprotein processing, depletion of Ca2+stores, acidosis, and hypoxia conditions that are also common in poorly vascularized tumor tissues.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD01322081
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 94 kDa
species reactivity human, canine, mouse
浓度 ~1 mg/mL
technique(s) immunocytochemistry: suitable
immunoprecipitation (IP): 10-20 μg using MDCK whole cell lysate
indirect immunofluorescence: 2-4 μg/mL using HeLa cells
microarray: suitable
western blot: 0.5-1 μg/mL using whole cell extracts of HeLa, MDCK, and NIH3T3 cells
UniProt登记号 P14625
运输 dry ice
储存温度 ?20℃
Gene Information human ... HSP90B1(7184)
mouse ... Hsp90b1(22027)
rat ... Tra1(298957)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Sigma-Aldrich

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