半岛bd体育手机客户端 说明

一般描述

Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.

应用

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal ofN-linked oligosaccharide chains and converts Asn residue to Asp.

生化/生理作用

Hydrolyzes an N⁴-(acetyl-β- D -glycosaminyl)asparagine in which the N-acetyl- D -glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β- D -glucoaminylamine and the peptide containing an aspartic residue.

单位定义

One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37℃.

外形

Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.

基本信息

半岛bd体育手机客户端 性质

安全信息