Lipase A Candida antarctica, recombinant fromAspergillus oryzae

半岛bd体育手机客户端 说明

一般描述

Lipase ACandida antarctica(CALA) belongs to the α/β hydrolase family. The catalytic triad comprises Ser184, His366, and Asp334 residues.

应用

Lipase ACandida antarctica, recombinant fromAspergillus oryzaehas been used in the enzyme-catalyzed hydrolysis studies with 3-acetylthiohexanal. It has also been used as a biocatalyst to test its effect on the esterification of phytosterol with caprylic acid.

生化/生理作用

Lipase ACandida antarctica(CALA) is a thermostable serine hydrolase with a highersn-2 preference towards triglycerides. It displays excellent activity towards large substrates as well as bulky sterically hindered tertiary alcohols.
Lipase A fromCandida Antarcticacatalyzes hydrolysis of tertiary alcohols to form glycerol and fatty acids and shows selectivity for the N-acylation of β-amino esters.

单位定义

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40℃ (triolein, Cat. No. 62314, as substrate); 1 U as described above is equivalent to ~0.15 U using trybutyrine, Cat. No. 91010, as substrate, at pH 8.0 and 70℃

其他说明

Characterization

基本信息

酶学委员会(EC)编号	3.1.1.3 ( BRENDA | IUBMB )
EC 号	232-619-9
MDL编号	MFCD00131509
NACRES	NA.26

半岛bd体育手机客户端 性质

形式	powder
质量水平	100
specific activity	~2 U/mg
保质期	limited shelf life, expiry date on the label
颜色	beige
储存温度	2-8℃

安全信息

储存分类代码	11 - Combustible Solids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)