Anti-HU-B27 Antibody, FITC conjugated

半岛bd体育手机客户端 说明

一般描述

HU-B27 (HLA- Com-B27) is involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.

特异性

This reagent binds strongly to the HLA-B27 antigen. It also binds weakly to the HLAB7 antigen. This formulation demonstrates less cross reactivity to HLA-B7 than Chemicon products, MAB1285 and MAB1285F.

应用

Detect HU-B27d using this Anti-HU-B27 Antibody, FITC conjugated validated for use in FC, IC, IF & WB.
Research Sub Category
Immunoglobulins & Immunology
Flow cytometry and Immunofluorescence: use 10 mL direct from the vial per 100 mL of whole blood, or 1 x 106 peripheral blood mononuclear cells (PBMC) in 100 mL buffer.

Flow cytometric analysis of lymphocyte staining for HLA-B27 gave the following results:
Subjects/Fluorescence Intensity
Heterozygous HLA-B27+/Strong
Homozygous HLA-B7+/Weak
Heterozygous HLA-B7+/Weak - negative
Non B27, Non B7/Negative

This reagent is useful in the investigation of HLA-B27 in diseases such as ankylosing spondylitis.

SUGGESTED USAGE:
Flow cytometry and immunofluorescence - use 10 ?l direct from the vial per 100 ?l of whole blood, or 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 ?l buffer.

Use Control IgG2a-FITC (MABC002F) as an isotype control.

Please note that this reagent should be used in a single-colour single-step assay only.
Research Category
Inflammation & Immunology

质量

Evaluated by Western Blot on Jurkat lysates.

Western Blotting Analysis:
1:500 dilution of this antibody detected Com-B27 on 10 ?g of Jurkat lysates.

目标描述

19 kDa

外形

The reagent is supplied in 1.0 mL phosphate-buffered saline, pH 7.4, containing 0.2% bovine serum albumin and 0.1% sodium azide. Purified from mouse ascitic fluid. The characteristics of each lot are tested by gel electrophoresis and flow cytometry.

储存及稳定性

Stable for 6 months at 2-8?C in the dark from date of receipt.

分析说明

Control
Control IgG2a-FITC (MABC002F) as an isotype control

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
偶联物	FITC conjugate
抗体形式	purified antibody
克隆	monoclonal
species reactivity	rat, mouse, human
manufacturer/tradename	Chemicon?
technique(s)	flow cytometry: suitable immunocytochemistry: suitable immunofluorescence: suitable western blot: suitable
同位素/亚型	IgG2a
UniProt登记号	P03989
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable