半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-c-Myc (mouse IgG1 isotype) is derived from the 9E10 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice. The antibody is isolated from ascites fluid and conjugated to fluorescein isothiocyanate isomer I (FITC). Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein. c-Myc is a proto-oncogene localized on long arm of human chromosome 8. The encoded protein contains an N-terminal transactivation domain.

特异性

Monoclonal Anti-c-Myc, FITC conjugate recognizes the c-Myc tag sequence on c-Myc tagged fusion proteins when expressed N- or C-terminal to the fusion protein.

免疫原

人p62^c-Myc蛋白的合成肽。

应用

Monoclonal Anti-c-Myc?FITC antibody produced in mouse has been used in immunofluorescence microscopy and immunocytochemistry.

生化/生理作用

c-Myc plays a vital role in regulation of cell growth, proliferation, differentiation and apoptosis. It is implicated in DNA replication, RNA splicing or transcription. Deregulation in the gene is associated with the development of various types of human cancers. An epitope located within amino acids 410-419, containing the sequence EQKLISEEDL of human c-Myc has been widely used as a tag in many expression vectors, enabling the expression of proteins as c-Myc tag fusion proteins. Epitope tags provide a method to localize gene products in a variety of cell types, to study the topology of proteins and protein complexes, and to identify associated proteins. In addition, it allows characterization of newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month.For extended storage, freeze in working aliquots.Repeated freezing and thawing is not recommended.Storage in “frost-free” freezers is not recommended. Ifslight turbidity occurs upon prolonged storage, clarifythe solution by centrifugation before use. Workingdilution samples should be discarded if not used within12 hours.

免责声明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses,in vitrodiagnostic uses,ex vivoorin vivotherapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.56

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	FITC conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	9E10, monoclonal
形式	buffered aqueous solution
species reactivity	human
储存条件	protect from light
增强验证	recombinant expression Learn more aboutAntibody Enhanced Validation
浓度	5 μg/mL (suitable for immunoflourescence)
technique(s)	immunocytochemistry: 5.0 μg/mL using mammalian cells transfected with c-Myc tagged fusion protein, fixed with paraformaldehyde.
UniProt登记号	P01106
运输	dry ice
储存温度	?20℃
Gene Information	human ... MYC(4609)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable