半岛bd体育手机客户端 说明

应用

Fusion proteins are commonly expressed with a factor Xa cleavable Ile-Glu (or Asp)-Gly-Arg-↓-X sequence. Typically 1 mg of fusion protein can be incubated with 10 μg of factor Xa for 2.5 hours at 37 ℃.

生化/生理作用

Factor Xa catalyzes the hydrolysis of the Arg-Thr and then Arg-Ile bonds in prothrombin to yield active thrombin.
The fairly strict recognition sequence is Ile-Glu (or Asp)-Gly-Arg-↓-X.
It may sometimes cleave at other basic residues, depending on the conformation of the target protein. Factor Xa will not cleave if a proline residue follows the arginine of the recognition sequence.
pH Optimum: 7.6-8.0
Temperature Optimum: 37 ℃

物理属性

Factor Xa is a serine endoproteinase and a member of the S1 peptidase family. Factor Xa plays a critical role in the coagulation cascade by catalyzing the proteolytic conversion of prothrombin to active thrombin. Factor Xa′s prothrombin conversion activity is greatly enhancedin vivowhen complexed with factor V, calcium ions and phospholipids on the activated platelet surface.
The zymogen form, Factor X, is activatedin vivoby two different pathways. The intrinsic pathway utilizes a catalytic complex composed of factor IXa, factor VIII, phospholipids and calcium ions. The extrinsic pathway utilizes a complex of factor VII and tissue factor. The factor X zymogen is a 55 KDa glycoprotein with a light and heavy chain joined by a single disulfide.

单位定义

One unit of activated Factor X will liberate 1.0 μmole ofp-nitroanilide from N-benzoyl- L -isoleucyl- L -glutamyl- L -glycyl- L -arginine-p-nitroaniline per minute at pH 8.3 at 37 ℃.

基本信息

酶学委员会(EC)编号	3.4.21.6 ( BRENDA | IUBMB )
MDL编号	MFCD00163610
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
形式	aqueous glycerol solution
specific activity	≥50 units/mg protein
UniProt登记号	P00743
运输	wet ice
储存温度	?20℃
Gene Information	cow ... F10(280787)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable