Fpg Protein fromEscherichia coli

半岛bd体育手机客户端 说明

一般描述

Fpg contains two domains separated by a flexible hinge.
Formamidopyrimidine-DNA glycosylase (Fpg) is a DNA repair enzyme found inEscherichia coli, which contains one zinc atom. Proximal to its C-terminal, it contains a zinc-finger motif of CC/CC type.

应用

Fpg Protein fromEscherichia colihas been used for the assessment of DNA oxidative damage using comet assay.

包装

10 μg in poly bottle

生化/生理作用

Fpg is a key enzyme in the DNA base excision repair pathway (BER). It catalyses the excision of a broad spectrum of modified purines. Fpg has both DNA glycosylase activity that removes the mutated base and AP-lyase activity that releases ribose leaving both 5′- and 3′-phosphorylated ends in the DNA. The zinc finger motif at its C-terminus is responsible for the DNA binding and AP-lyase activity. In addition, its N-terminal proline acts as a nucleophile to produce a Schiff base intermediate that is essential for enzyme action.
Fpg specifically acts on 3′- and 5′-phosphodiester bonds.
Formamidopyrimidine-DNA glycosylase (Fpg) cleaves double-stranded DNA containing the damaged base 8-oxo-7,8-dihydroguanine. It functions as anN-glycosylase and apurinic/apyrimidinic lyase.

单位定义

One unit will cleave 50% of 0.5 pmol of double-stranded DNA oligomer substrate (8-oxoguanine?mutated) in 10 min at 25 ℃.

外形

Solution in 50% glycerol containing 50 mM potassium HEPES, pH 7.5, 1 mM DTT, 1 mM EDTA, and 200 mM NaCl.

基本信息

酶学委员会(EC)编号	3.2.2.23 ( BRENDA | IUBMB )
MDL编号	MFCD02264758
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	200
生物来源	Escherichia coli
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	buffered aqueous glycerol solution
specific activity	>20,000 units/mg protein
分子量	30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
UniProt登记号	P05523
application(s)	genomic analysis
运输	wet ice
储存温度	?20℃
Gene Information	Escherichia coliCFT073 ... mutM(1038243) Escherichia coliK12 ... mutM(946765)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 2
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)