半岛bd体育手机客户端 说明

一般描述

2× 浓缩的即用型热启动预混液，用于 qPCR 和 qRT-PCR，使用实时 PCR 半岛游戏平台官网入口网址 上的水解探针检测格式。

EagleTaq Universal Master Mix (ROX) 是一种即用型 2× 浓缩 PCR Master Mix，包含了进行定量、实时 PCR 水解探针反应所需的所有试剂(引物、探针和模板除外)。它包含一种特殊的 ROX 引用染料，使其适用于所有需要使用 ROX 引用染料进行定量分析的实时半岛游戏平台官网入口网址 。
EagleTaq Universal Master Mix (ROX) 含有 dUTP，因此可与尿嘧啶-DNA 糖基化酶 (UNG) 配合使用，以防止携带污染引起的假阳性。
热启动属性允许在环境温度下设置反应。
有了这种耐用的试剂，任何 PCR 方案优化都能最小化
The EagleTaq Universal Master Mix (ROX) is a ready-to-use, 2× concentrated polymerase chain reaction (PCR) master mix that contains all the reagents (except primers, probes, and template) needed for performing quantitative, real-time PCR (qPCR) and quantitative reverse transcription PCR (qRT-PCR) hydrolysis probe reactions.

应用

EagleTaq Universal Master Mix (ROX) has been used in quantitative, real-time polymerase chain reaction (qPCR ) and quantitative reverse transcription PCR (qRT-PCR).

特点和优势

• The EagleTaq Universal Master Mix (ROX) contains a special ROX reference dye which makes it suitable for all real-time instruments on which a ROX reference dye is needed for quantitative analysis.
• It contains dUTP so that it may be used with Uracil-DNA Glycosylase (UNG) to prevent false positives arising from carryover contamination.
• The hot start properties allow reaction setup at ambient temperature.
• With this robust reagent, any PCR protocol optimization is minimized.

组分

含有 EagleTaq DNA 聚合酶和 dATP、dCTP、dGTP、dUTP、MgCl₂和 ROX 的反应缓冲液。

半岛bd体育手机客户端 性质

质量水平	100
用途	sufficient for 100 reactions sufficient for 500 reactions sufficient for 5000 reactions
特点	dNTPs included hotstart
manufacturer/tradename	Roche
包装	pkg of 100 x 20 μL reactions (07260288190) pkg of 500 x 20 μL reactions (07260296190) pkg of 5000 x 20 μL reactions (07249926190)
technique(s)	RT-qPCR: suitable qPCR: suitable
input	purified DNA
detection method	probe-based

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	does not flash
闪点(C)	does not flash