High Pure PCR Cleanup Micro Kit

半岛bd体育手机客户端 说明

一般描述

Nucleic acids bind to the surface of the glass fiber fleece in the presence of a chaotropic salt (guanidine HCl). This allows the High Pure filter tube to specifically immobilize nucleic acids (both DNA and RNA) while they are freed of contaminants. The amount of DNA recovered is dependent on the amount of DNA applied to the glass fiber fleece, the elution volume, and the length of the amplification/DNA products. When 5 - 25 μg DNA is applied to the kit′s High Pure Micro Filter Tube, approximately 85% of the DNA can be recovered.

Capacity of High Pure Micro Filter Tubes:The High Pure Micro Filter Tubes hold up to 500 μL sample volume.
The High Pure PCR Cleanup Micro Kit efficiently purifies products from PCR and other reactions. The kit eliminates primers, mineral oil, salts, unincorporated nucleotides, and thermostable DNA polymerases, which may inhibit subsequent enzymatic reactions such as labeling, sequencing, or cloning of PCR products.

应用

The High Pure PCR Cleanup Micro Kit is used to isolate PCR products from amplification and other reactions, and can be used in many molecular biology applications:

• Restriction enzyme digests
• Alkaline phosphatase treatment
• Kinase reactions
• Nonradioactive labeling

The kit can also be used to:

• Purify cDNA
• Concentrate dilute nucleic acid solutions
• Recover DNA from agarose gel slices

Use one kit for a variety of applications.
The fast and simple High Pure protocols use a tabletop centrifuge to bind, wash, and elute the reaction product down to 10 μL (micro format) in as little as 10 minutes. The procedure conveniently eliminates a concentration step, and is ideal for downstream applications such as labeling, sequencing, cloning, ligation, or amplification using PCR. The purified DNA can also be used for Southern blotting and in vitrotranscription.

特点和优势

• Conserve resources and save time

by using a single kit with a simple, rapid protocol.

• Obtain purified product in a small elution volume

(≤10 μL) for demanding downstream applications.

• Generate contaminant-free DNA

for direct use in cloning, ligation, restriction digests, and other reactions.

• Selectively isolate specific DNA fragment sizes

by using the kit′s Binding Enhancer to adjust purification stringency.

• Eliminate the use of hazardous organic compounds

such as cesium chloride, phenol, chloroform, and ethidium bromide

组分

• Binding Buffer
• Binding Enhancer
• Wash Buffer Concentrate
• Elution Buffer
• High Pure Micro Filter Tubes
• Collection Tubes

质量

Greater than 70% recovery is obtained when 3 μg Roche DNA Molecular Weight Marker VI is applied to the kit′s High Pure Micro Filter Tubes. Gel electrophoresis of the eluate after purification confirms the complete removal of primer-dimers. The eluted, purified DNA shows no inhibition of amplification using a LightCycler^?Instrument with the LightCycler^?FastStart DNA Master^PLUSSYBR Green I.

制备说明

Nucleic acids bind specifically to the surface of glass fibers in the presence of chaotropic salts. Since the binding process is specific for nucleic acid, the bound material can be separated and purified from impurities by a simple wash step. The Binding Enhancer enables the modification of DNA fragment size exclusions. Small oligonucleotides and dimerized primers from amplification reactions are selectively removed. The nucleic acids elute from the glass fiber fleece in a low-salt buffer or water.

分析说明

A 341 bp PCR fragment of the tPA gene was amplified according to a standard block cycler protocol. The resulting reaction mixes were pooled and purified with the High Pure PCR Cleanup Micro Kit. Different amounts of Binding Enhancer were used in the purification procedure. Portions of the PCR product (250 ng each, lanes 2 – 5) and the PCR product mix (16 μL each, lanes 6 – 7) were analyzed on a 1% agarose gel (see Figure 1).
The yield from each purification is shown in Table 1.

其他说明

仅用于生命科学研究。不可用于诊断。
High Pure Technology and Silica Adsorption Kits

法律信息

LightCycler is a registered trademark of Roche

半岛bd体育手机客户端 性质

用途	sufficient for 200 purifications (04983912001) sufficient for 50 purifications (04983955001)
质量水平	100
manufacturer/tradename	Roche
包装	pkg of 200 purifications (04983912001) pkg of 50 purifications (04983955001)
technique(s)	DNA purification: suitable

安全信息

象形图
警示用语：	Danger
危险声明	H302 + H332 - H314 - H360FD - H412
预防措施声明	P201 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338 + P310 - P308 + P313
危险分类	Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 1B - Skin Corr. 1B
补充剂危害	EUH019 - EUH032
储存分类代码	6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK	WGK 2
闪点(F)	285.8 °F
闪点(C)	141 ℃