pBICEP-CMV^TM-3 Expression Vector

半岛bd体育手机客户端 说明

一般描述

The pBICEP-CMV-3 bicistronic expression vector is a 5.3 kb derivative of pCMV5used for transient or stable co-expression of an N-terminal 1? FLAG? fusion protein and a selection gene in mammalian cells.

包装

20 μg in poly bottle

应用

For transient, cytoplasmic expression of an N-terminal FLAG^?fusion and a second gene of interest or selection marker from bicistronic mRNA. Two genes can be cloned into MCS1 and MCS2 for transcription of a single message from the CMV promoter. This vector is useful for protein-protein interaction studies, multi-subunit proteins, and cloning a selection marker of choice.

生化/生理作用

The presence of two multiple cloning sites not only allows the user to clone a target gene, but a selectable marker of their choice, as well. The promoter-regulatory region of the human cytomegalovirus immediate early promoter drives transcription of the FLAG-fusion construct along with a downstream selection gene. The EMCV IRES region controls translation of the selection gene by recruiting the ribosomal subunits for cap-independent translational initiation. Depending on the mode of selection, stable transfectants can be generated by transfection using the appropriate ESCORT? product line specific for the cell type.

其他说明

您可在我们的FLAG^?文献门户上浏览更多应用文献。

法律信息

FLAG is a registered trademark of sigma-aldrich Co. LLC
pBICEP-CMV is a trademark of sigma-aldrich Co. LLC

半岛bd体育手机客户端 性质

质量水平	200
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves