Epiregulin from mouse

半岛bd体育手机客户端 说明

生化/生理作用

Binds and activates the tyrosine kinase ErbB family receptors (ErbB1-ErbB4). Inhibits the growth of several epithelial tumor cells and stimulates the growth of fibroblasts and other cell types. Its expression is upregulated in a number of carcinoma cell lines.

外形

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 0.5 mg bovine serum albumin.

制备说明

Recombinant Mouse Epiregulin is produced from a DNA sequence encoding the mature mouse epiregulin protein sequence. The protein is expressed inE. coli.

Epiregulin is a member of EGF (epidermal growth factor) family. All members of the EGF family are synthesized as transmembrane precursors and converted to soluble forms by proteolytic cleavage. Epiregulin was originally purified from the mouse fibroblast-derived tumor cell line NIH3T3/T7. Mouse epiregulin cDNA encodes a transmembrane precursorof 162 amino acids with a mature soluble form consisting of residues 56-101. Epiregulin acts like other EGF family members by binding to and activating the tyrosine kinase ErbB family receptors (ErbB1-ErbB4). Epiregulin has a broad specificity, but this ligand seems to preferentially activate heterodimeric receptor complexes. Epiregulin inhibits the growth of several epithelial tumor cells and stimulates the growth of fibroblasts and other types of cells. Its expression is upregulated in a number of carcinoma cell lines. Epiregulin is also an autocrine growth factor in human epidermal keratinocytes, and involved in the early stages of pregnancy, regulating the attachment of the blastocyst to the uterine epithelium during the implantation process.

分析说明

The biological activity is measured by its ability to stimulate the³H-thymidine incorporation in a mouse fibroblast cell line, Balb/3T3.

基本信息

MDL编号

MFCD06798643

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
测定	≥97% (SDS-PAGE)
形式	lyophilized powder
分子量	predicted mol wt ~5.5 kDa
technique(s)	cell culture | mammalian: suitable
杂质	endotoxin, tested
UniProt登记号	Q61521
储存温度	?20℃
Gene Information	mouse ... Ereg(13874)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)