Interferon-γ Protein, Recombinant mouse

半岛bd体育手机客户端 说明

一般描述

Product Source: Mouse, expressed in E.coli
Interferon-gamma (IFN-gamma) is a regulatory protein produced by activated NK cells and CD4+TCRalpha/beta+, CD8+TCRalpha/beta+, and TCRgamma/delta+ T cells. The biological activities of IFN-gamma include the specific binding to a single class of high affinity receptors. In addition, IFN-gamma can upgrade the number of lymphoid cell functions including the antimicrobial and antitumor responses of macrophages, NK cells, and neutrophils. Recombinant mouse IFN-gamma is a 15.6 kDa protein containing 134 amino acid residues.

应用

For most in vitro applications, IFN-gamma exerts its biological activity in the concentration range of 0.1 to 10.0 ng/mL.

生化/生理作用

The ED50 as determined by a cytopathic effect inhibition assay with mouse NIH 3T3 cells challenged VSV is ≤ 0.1 ng/mL

外形

Sterile filtered, lyophilized from 10mM Sodium Phosphate, pH 7.0 + 25mM NaCl.

储存及稳定性

The lyophilized protein is stable for a few weeks at room temperature, but is best stored at -20°C. After a quick spin, reconstitute with Sodium Phosphate, pH 8.0 to a concentration of 1 mg/mL. Further dilutions should be made into RPMI + 10% FCS or 5% HAS and stored at 2-8?C for one week or -20?C for future use. Reconstituted IFN-gamma should be stored in undiluted aliquots at -20°C for up to six months from date of receipt.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160405

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
测定	>98% (SDS-PAGE and HPLC)
specific activity	≥1.0 x 10E7 U/mg
manufacturer/tradename	Chemicon?
technique(s)	cell culture | mammalian: suitable
杂质	<0.1 ng/μg endotoxin (of IFN-gamma; 1EU/μg)
input	sample type mesenchymal stem cell(s) sample type hematopoietic stem cell(s)
UniProt登记号	P01579 P01580
运输	dry ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable