Anti-dimethyl-Histone H3 (diMe-Lys⁹) antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Histone methylation is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis and heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9 and Lys27 are the preferred sites of methylation. SET domain-, site-specific histone methyltransferases (HMTases) are involved in methylation of Lys9 in histone 3.

特异性

ChIP validated

免疫原

synthetic methylated peptide corresponding to amino acids 5-13 (diMe-Lys⁹) of human histone H3, conjugated to KLH. This sequence is identical in many species including mouse, rat, bovine, chicken, frog,Drosophila,C. elegans, tetrahymena, andArabidopsis thalianahistone H3.

应用

Anti-dimethyl-Histone H3 (diMe-Lys⁹) antibody produced in rabbit has been used in immunoblotting and chromatin immunoprecipitation assay.

生化/生理作用

Mono- and dimethylation of H3 at Lys9 are intrinsically linked to epigenetic silencing and heterochromatin assembly. Methylation of H3 at Lys9 generates a binding site for HP1 proteins, a family of heterochromatic adaptor proteins implicated in both gene silencing and in the organization of higher order chromatin.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD06798437
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	200
偶联物	unconjugated
抗体形式	IgG fraction of antiserum
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen 17 kDa
species reactivity	bovine, human,Drosophila,Arabidopsis thaliana, rat, frog, chicken,Caenorhabditis elegans, mouse
technique(s)	ChIP: suitable microarray: suitable western blot: 1:1,000-1:2,000 using whole extract of human epitheloid carcinoma HeLa cell line
UniProt登记号	P68431
运输	dry ice
储存温度	?20℃
Gene Information	human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290) mouse ... H3c10(319152), H3c11(319153), H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114) rat ... H3f3b(117056)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable