Dextran Sucrase fromLeuconostoc mesenteroides

半岛bd体育手机客户端 说明

一般描述

Dextran Sucrase fromLeuconostoc mesenteroidesbelongs to glycoside hydrolase family 70 (GH70). It functions through a retaining mechanism and uses two catalytic acidic residues. Dextran sucrase has a dextran binding site in the C-terminal domain.

应用

Dextran sucrase from Leuconostoc mesenteroides has been used in a study to investigate the functional and structural characterization of α-(1→2) branching sucrase derived from DSR-E glucansucrase. Dextran sucrase from Leuconostoc mesenteroides has also been used in a study to investigate the bioengineering of Leuconostoc mesenteroides glucansucrases.
The enzyme from Sigma has been used to prepare immobilized sphere for the production of dextran from sucrose.
Dextran Sucrase fromLeuconostoc mesenteroideshas been used:

• in immobilization on shirasu porous membrane (SPG) for dextran production
• in the enzymatic synthesis of dextran nanoparticles at various pH range
• in the immobilization with hydroxyapatite for dextran production

包装

10 units in glass bottle

生化/生理作用

Dextransucrases are glucansucrases that are able to produce dextran, a glucose polymer linked mainly through α1-6 bonds. However, α1-3, α1-6, α1-4 and α1-2 bonds are also found, in both the main chain and the branching linkages. The peptide has approximately 1600 amino acids. The aspartic acid in position 551 is essential for catalytic activity, while glutamic acid 589 and aspartic acid 662 complement the catalytic triad. The activity of dextransucrase is decreased by EDTA, and is restored by the addition of calcium ions. Zinc, cadmium, lead, mercury and copper ions are inhibitory to various degrees.

质量

色谱纯化半岛bd体育手机客户端

单位定义

One unit will liberate 1.0 μmole of fructose per min at 37 ℃, pH 5.4.

外形

Lyophilized powder containing dextran, MES buffer salts and CaCl₂

基本信息

酶学委员会(EC)编号	2.4.1.5 ( BRENDA | IUBMB )
MDL编号	MFCD00130936
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	200
specific activity	≥100 units/mg protein
组成	Protein, ~15% Lowry
溶解性	H2O: soluble 0.9-1.1 mg/mL, clear to slightly hazy, colorless to light yellow
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)