Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Cy3/Cy5 (mouse IgG2a isotype), Biotin conjugate is derived from the CY-96 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a mixture of proteins labelled with cyanine 3 (Cy3) or Cy5. Cyanine dyes are ionized polymethine dyes.

特异性

The antibody recognizes Cy3 and Cy5 conjugated to proteins.

免疫原

mixture of proteins labeled with Cy3/Cy5.

应用

Monoclonal Anti-Cy3/Cy5 Biotin antibody produced in mouse has also been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunocytochemistry
• dot blot assay
• protein microarrays
• immunofluorescenc

Monoclonal Anti-Cy3/Cy5-Biotin antibody produced in mouse was used to label miR-107 in human breast cancer cell lines by confocal microscopy.

生化/生理作用

Cy dyes are pH insensitive fluorophores that are used to label, DNA, RNA and proteins in a variety of biological assays.
Cyanine dyes are small, soluble in aqueous solution and has tolerance to dimethyl sulfoxide (DMSO). They are more photostable than fluorescein, has high molar extinction coefficients and favourable quantum yields. Monoclonal antibodies to cyanine 3 (Cy3) and/or Cy5 are an important tool for Cy3/Cy5 signal amplification in assays where biomolecules are labelled with Cy3 or Cy5.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.46

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	biotin conjugate
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	CY-96, monoclonal
形式	buffered aqueous solution
浓度	~1 mg/mL
technique(s)	direct ELISA: suitable dot blot: suitable immunocytochemistry: 1-2 μg/mL using chicken fibroblasts microarray: suitable
同位素/亚型	IgG2a
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)