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位置: 首页> 品牌> Sigma-Aldrich> Anti-Caspase 9 antibody,Mouse monoclonal

Anti-Caspase 9 antibody,Mouse monoclonal

品牌
Sigma-Aldrich
货号
C4356
规格纯度
clone CAS9, purified from hybridoma cell culture
价格
5531.3 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-Caspase 9 antibody,Mouse monoclonal
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.

免疫原

synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.

应用

Anti-Caspase 9 antibody,Mouse monoclonal has been used in:

  • western blot assay
  • immunocytochemistry
  • enzyme-linked immunosorbent assay (ELISA).

生化/生理作用

Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD01864176
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 CAS9, monoclonal
形式 buffered aqueous solution
分子量 antigen 37 kDa
species reactivity human
浓度 ~2 mg/mL
technique(s) indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation
同位素/亚型 IgG1
UniProt登记号 P55211
运输 dry ice
储存温度 ?20℃
Gene Information human ... CASP9(842)

安全信息

储存分类代码 10 - Combustible liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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