Cholesterol Dehydrogenase fromNocardiasp.

半岛bd体育手机客户端 说明

应用

The enzyme sequence is derived fromNocardia sp., and this cholesterol dehydrogenase is expressed inStreptomyces lividans. Cholesterol Dehydrogenase fromNocardiasp. has been used in the preparation of amperometric cholesterol biosensor for cholesterol detection in biological samples and in a study to investigate the atomic structure of the autosomal recessive hypercholesterolemia phosphotyrosine-binding domain with the LDL-receptor tail. Cholesterol dehydrogenase fromNocardia sp.Has also been used in a study to assess organization, dynamics, and segregation of Ras nanoclusters in membrane domains.
Enzyme used in determining cholesterol in serum

包装

500 units in poly bottle

生化/生理作用

Cholesterol dehydrogenase (CDH) is a nicotinamide adenine dinucleotide phosphate (NAD(P)) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Cholesterol detection by using CDH allows direct and easier quantification compared to cholesterol oxidase based assay.
Cholesterol dehydrogenase is a NAD(P) dependent microbial dehydrogenase that oxidizes the 3-β-hydroxyl group of cholesterol to yield cholest-4-ene-3-one. Molecular mass of the protein is found to be 37 kDa based on SDS-PAGE. Its pH is 4.5.

单位定义

One unit produces 1.0 μmole of cholesten-4-en-3-one per minute at pH 8.5 at 25 ℃.

外形

Supplied as a lyophilized powder

基本信息

酶学委员会(EC)编号	1.1.1.B40
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inStreptomyces lividans
形式	lyophilized solid
specific activity	≥18 unit/mg solid
分子量	~37 kDa by SDS-PAGE
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H334
预防措施声明	P261 - P284 - P304 + P340 + P312 - P501
危险分类	Resp. Sens. 1
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)