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Protein Kinase A catalytic subunit human

品牌
Sigma-Aldrich
货号
C8482
规格纯度
>90% (SDS-PAGE), recombinant, expressed inE. coli, buffered aqueous glycerol solution
价格
11383.1 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Protein Kinase A catalytic subunit human
Cyclic AMP-dependent protein kinase catalytic subunit, histidine-tagged, recombinant human
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

生化/生理作用

Ubiquitous serine-threonine kinase that phosphorylates a broad spectrum of substrates, and regulates many cellular processes. The catalytic subunit is released following binding of cyclic AMP to the regulatory subunits of the PKA holoenzyme. The free catalytic subunit has intrinsic activity and does not require added cyclic AMP.

外形

Solution in 30 mM potassium phosphate buffer, pH 7.4, containing 50% glycerol, 150 mM KCl, 1 mM EDTA, and 1 mM DTT.

分析说明

One unit will transfer 1 nanomole of phosphate from32P-ATP to kemptide substrate per minute at pH 7.4 at 30 ℃ (measured by a radioactive filter-binding assay).
Km(ATP) = 25 μM at 30 ℃, pH 7.4. Km(kemptide) = 42 μM (33 μg/ml) at 30 ℃, pH 7.4.

基本信息

MDL编号 MFCD03458215
NACRES NA.32

半岛bd体育手机客户端 性质

质量水平 200
重组 expressed inE. coli
测定 >90% (SDS-PAGE)
形式 buffered aqueous glycerol solution
specific activity >1000 units/mg protein
分子量 43.5 kDa
UniProt登记号 P17612
运输 dry ice
储存温度 ?70℃
Gene Information human ... PRKACA(5566)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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