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CMP-Sialic Acid Synthetase fromNeisseria meningitidisgroup B

品牌
Sigma-Aldrich
货号
C1999
规格纯度
recombinant, expressed inE. coliBL21, ≥10 units/mg protein
价格
3863.36 *本价格含增值税费
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半岛bd体育手机客户端 名称:
CMP-Sialic Acid Synthetase from Neisseria meningitidisgroup B
CTP: N -Acylneuraminate cytidylyltransferase
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Cytidine monophosphate (CMP)-Sialic Acid Synthetase fromNeisseria meningitidisgroup B is encoded in neuA gene. The protein has a molecular weight of 24.8 kDa.

应用

The enzyme has been utilized to synthesize CMP-sialic acid and its derivatives.

生化/生理作用

Cytidine monophosphate (CMP)-sialic acid synthetase catalyses the conversion ofN?acetylneuraminic acid (NeuNAc) to CMP-NeuNAc. CMP-sialic acid synthetase has globular α/β domain and is categorised under αβα three-layered sandwich fold. The dimerization domain aids the interaction between the monomers. It also has mononucleotide binding and NeuAc binding pocket. Mg2+is essential for the catalytic functionality of CMP-sialic acid synthetase.

单位定义

One unit will catalyze the formation of 1 μmol CMP-Neu-5-Ac from Neu-5-Ac and CTP per minute at 37 ℃ at pH 8.0.

外形

Supplied as a lyophilized powder containing Tris-HCl and NaCl.

分析说明

Enzymatic activity assays are performed in Tris-HCl buffer (100 mM, pH 8.5) containing Neu-5-Ac (1 mM) and CTP (1 mM) at 37 ℃ for 30 min and analyzed using capillary electrophoresis with a UV detector (200 nm).

基本信息

酶学委员会(EC)编号 2.7.7.43 ( BRENDA | IUBMB )
MDL编号 MFCD01323074
NACRES NA.54

半岛bd体育手机客户端 性质

质量水平 200
重组 expressed inE. coliBL21
形式 lyophilized solid
specific activity ≥10 units/mg protein
分子量 26.0 kDa
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 13 - Non Combustible Solids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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