半岛bd体育手机客户端 说明

应用

Human carbonic anhydrase II has been used in a study to assess quantitative imaging of mitochondrial and cytosolic free zinc levels in an in vitro model of ischemia/reperfusion. Human carbonic anhydrase II has also been used in a study to investigate a new scrubber concept for catalytic CO₂hydration.

生化/生理作用

Carbonic anhydrase is a zinc metalloenzyme that catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in vital physiological and pathological processes such as pH and CO₂homeostasis, transport of bicarbonate and CO₂, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. It is required for renal acidification. Its absence can lead to osteoporosis, renal tubular acidosis, and cerebral calcification. Therefore, this enzyme is an important target for inhibitors with clinical applications in glaucoma, epilepsy and Parkinson′s disease. In addition, it is being explored as a potential target for obesity and cancer. CAII has a molecular mass of approximately 30 kDa and is primarily present in type II pneumocytes. Due to this unique location it has been speculated that CAII is involved in pulmonary functions such as regulation of fluid secretion and facilitation of CO₂elimation. Sulfonamides, sulfamates and sulfamides are potent inhibitors of CA.

单位定义

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 ℃. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

外形

Supplied as a solution in 20 mM Tris, pH 7.5, with 150 mM NaCl.

基本信息

酶学委员会(EC)编号	4.2.1.1 ( BRENDA | IUBMB )
MDL编号	MFCD01634577
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
形式	buffered aqueous solution
specific activity	≥5,000 units/mg protein
分子量	large subunit 17-22 kDa small subunit 10-12 kDa
浓度	800-1000 μg/mL
NCBI登记号	NM_000067 NP_000058
运输	wet ice
储存温度	?20℃
Gene Information	human ... CA2(760)

安全信息

储存分类代码

12 - Non Combustible Liquids