Anti-Cathepsin L antibody, Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Anti-Cathepsin L antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human procathepsin L.
Monoclonal Anti-Cathepsin L (mouse IgG1 isotype) is derived from the hybridoma CPL33/1 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from BALB/c mice immunized with human procathepsin L.

特异性

The antibody recognizes the native and denaturated forms of the protein and does not cross react with human cathepsin V. Monoclonal Anti-Cathepsin L specifically recognizes human cathepsin L (～ 25 kDa) and procathepsin L (～ 42 kDa). Anti-Cathepsin L antibody epitope resides within amino acids of human cathepsin L (FYKE).

免疫原

human procathepsin L. The antibody epitope resides within amino acids 258-261 of human cathepsin L (FYKE).

应用

Anti-Cathepsin L antibody has been used for immunoblotting.
Monoclonal Anti-Cathepsin L antibody produced in mouse is suitable for:

• immunohistochemistry
• indirect ELISA
• western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells

生化/生理作用

Inhibition of the enzyme or the proenzyme by low molecular weight inhibitors or by specific antibodies led to a suppression of the invasive capabilities of malignant cells, or a decline in their ability to form tumors in experimentalin vivoandin vitromodels.
Cathepsins are lysosomal proteases that play an important role in the intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01321805
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	CPL33/1, monoclonal
形式	buffered aqueous solution
分子量	antigen ~25 kDa (human cathepsin L) antigen ~42 kDa (human pro cathepsin L)
species reactivity	human
浓度	~2 mg/mL
technique(s)	immunohistochemistry : suitable indirect ELISA : suitable western blot : 0.1-0.2 μg/mL using total cell extracts of A549 cells
同位素/亚型	IgG1
运输	dry ice
储存温度	?20℃
Gene Information	human ... CTSL1(1514)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter