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Anti-Cathepsin B antibody, Mouse monoclonal

Monoclonal Anti- Cathepsin B 小鼠抗

品牌
Sigma-Aldrich
货号
C6243
规格纯度
clone CB59-4B11, purified from hybridoma cell culture
价格
6157.72 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-Cathepsin B antibody, Mouse monoclonal
Monoclonal Anti- Cathepsin B 小鼠抗
Anti-CYP1A2
Anti-CP12
Anti-P450(PA)
Anti-P3-450
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

The CTSB (Cathepsin B) gene encoding cathepsin B is mapped to human chromosome 8p23.1. Cathepsin B are normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell that take part in local proteolysis. Cathepsin B is both an endo- and exopeptidase, composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W

免疫原

Recombinant human cathepsin B.

应用

indirect ELISA, immunohistochemistry, In HCT-116 cells. 4-8μg/mL of antibody concentration may be used. A dilution of 1:800 in human pancreatic carcinoma cell line PANC-1 was used for immunoblotting

生化/生理作用

Cathepsins are lysosomal proteases activation of enzyme precursors, and tumor invasion and metastasis. It is involved in the proteolysis of many proteins involved in different physiological and pathological pathways. In the brain, this protease, also known as amyloid precursor protein secretase, is responsible for the proteolytic processing of amyloid precursor protein (APP). An incomplete proteolytic processing of APP has been found as a causative factor in Alzheimer disease. Furthermore, ablated genes coding cathepsin B and L leads to enormous neuronal cell death,probably due to autophagy abnormalities. Cathepsin B has been implicated in tumor progression by virtue of localization at the invading front of the tumor. For example, blocking cathepsin B expression in human glioblastoma SNB19 cells by a stable transfection of an expression vector expressing antisense cathepsin B, caused suppression of glioblastoma-induced neovascularization.
Monoclonal Anti-Cathepsin B specifically recognizes human1 and rat1 cathepsin B (~ 25 kDa). The antibody epitope resides within the peptide sequence EPGYSP which corresponds to amino acids 212-217 of human cathepsin B. The antibody does not cross react with the closely related lysosomal cysteine proteases: cathepsins L, H, K, S, V, and W.
Cathepsins significantly takes part in the immune response, by processing antigens into peptides for its presentation through MHC class II molecules.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 CB59-4B11, monoclonal
形式 buffered aqueous solution
分子量 antigen ~25 kDa
species reactivity human, rat
浓度 ~1.5 mg/mL
technique(s) immunohistochemistry: suitable
indirect ELISA: suitable
western blot : 4-8 μg/mL using HCT-116 cells.
同位素/亚型 IgG1
UniProt登记号 P07858
运输 dry ice
储存温度 ?20℃
Gene Information human ... CTSB(1508)
rat ... CTSB(64529)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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