Clostripain fromClostridium histolyticum

半岛bd体育手机客户端 说明

应用

Clostripain fromClostridium histolyticumhas been used as a proteolytic enzyme in perfusate to detect its effect on tube hematocrit. It has also been used in limited proteolysis of DNA polymerase (gp43) of phage T4 (RB69 gp43).

生化/生理作用

Clostripain fromClostridium histolyticumis composed of two polypeptide chains, with molecular masses of 41.7 kDa and 15.4 kDa. Clostripain has a highly restricted substrate specificity for Arg-Xaa peptide bonds. Therefore, clostripain has been explored as a potential enzyme for protein sequencing purposes. It has also been studied as a catalyst for condensation of pharmaceutically important peptides containing Arg-Pro bonds.

包装

Bottomless glass bottle. Contents are inside inserted fused cone.

质量

Purified, essentially salt-free

单位定义

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 7.6 at 25 ℃ in the presence of 2.5 mM DTT.

分析说明

The enzyme must be activated for 2-3 hours before use by dissolving in 2.5 mM dithiothreitol containing 1.0 mM calcium acetate.

基本信息

酶学委员会(EC)编号	3.4.22.8 ( BRENDA | IUBMB )
EC 号	232-822-2
MDL编号	MFCD00130814
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	100
specific activity	≥20 units/mg solid
分子量	15.4 kDa 41.7 kDa
杂质	salt, essentially free
储存温度	2-8℃

安全信息

象形图
警示用语：	Danger
危险声明	H315 - H319 - H334 - H335
预防措施声明	P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
危险分类	Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官	Respiratory system
储存分类代码	11 - Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)