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Anti-CD28 antibody, Mouse monoclonal

品牌
Sigma-Aldrich
货号
C7831
规格纯度
clone CD28.2, purified from hybridoma cell culture
价格
4731.57 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-CD28 antibody, Mouse monoclonal
Monoclonal Anti-CD28
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

CD28 (Cluster of differentiation 28) is a glycosylated membrane protein. The protein exists as a homodimer. CD28 is encoded by the gene mapped to human chromosome 2q33. The encoded protein is a co-stimulatory receptor expressed on naive T-cells. CD28 is characterized with a short cytoplasmic tail and two proline-rich motifs.

特异性

Recognizes the CD28 (44 kDa) human cell surface disulfide-linked, homodimeric glycoprotein. The antibody reacts with human CD28-transfected murine T-cell hybridoma, PHA-activated blasts and IL-6 dependent plasmacytoma lines. It cross reacts with rhesus monkey PBL. The antibody blocks CD28/B7 (CD80) mediated cell-cell adhesion. It binds to the CDR domain in a CDR1 dependent manner. It enhances CD3 monoclonal antibody-induced T-cell proliferation. May be used in adhesion and co-stimulation studies.
5th Workshop: code no. CD28.05.

免疫原

human CD28 transfected murine T cell hybridoma.

应用

Monoclonal Anti-CD28 antibody produced in mouse is suitable for the following applications:

  • Flow cytometry at a dilution of 5μL using 1 × 106 cells
  • Immunocytochemistry
  • Immunohistochemistry (frozen sections)
The antibody has been used for development of an assay to identify small molecules antagonists of CD28/CD86 binding.

生化/生理作用

CD28 (Cluster of differentiation 28) is a protein expressed on T-cells that provide co-stimulatory signals required for T-cell activation, survival and differentiation into effector T cells. CD28 is also expressed on multiple myeloma (MM) cells and is a key mediator of MM survival and apoptotic resistance. CD28 pro-survival signaling is dependent upon downstream activation of phosphatidylinositol 3-kinase (PI3K)/AKT (RAC-alpha serine/threonine-protein kinase), inactivation of the transcription factor FoxO3a and decreased expression of the pro-apoptotic molecule BIM. CD28 plays an important role as a mediator of MM survival during stress and can be targeted to overcome chemotherapy resistance. Inflammatory CD28-T-cells are found to be accumulated in livers of patients with primary sclerosing cholangitis (PSC) and are localized around bile ducts. Autoantibodies against CD28 are found in patients with autoimmune and atopic diseases.

目标描述

CD28 is expressed on thymocytes, peripheral lineage cell subsets, activated B cells and plasma cells. The antigen is involved in adhesion between T and B lymphocytes through the B7/BB-1 ligand expressed on activated B cells. CD28 co-stimulates T-cells activated via the CD3 T-cell receptor and CD2 pathways and induces their proliferation.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 CD28.2, monoclonal
形式 buffered aqueous solution
分子量 antigen 44 kDa
species reactivity rhesus monkey, human
technique(s) flow cytometry: 5 μL using 1 × 106cells
immunocytochemistry: suitable
immunohistochemistry (frozen sections): suitable
同位素/亚型 IgG1
UniProt登记号 P10747
运输 wet ice
储存温度 2-8℃
Gene Information human ... CD28(940)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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