半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Cathepsin K (mouse IgG2b isotype) is derived from the hybridoma CK124-1H6 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with recombinant human cathepsin K.
Cathepsins are proteases that normally localized in lysosomes of almost all mammalian cells, but under certain conditions they can be secreted from the cell. Cathepsins are classified based on their functions and presence of active site such as cathepsin B, D, K, V, F etc.

特异性

The antibody does not cross react with the closely related lysosomal cysteine proteases cathepsins L, B, H, S, V and W.

免疫原

recombinant human cathepsin K. The antibody epitope resides within the peptide sequence RGYREIPEGNEKAL that corresponds to amino acids 222-235.

应用

Monoclonal Anti-Cathepsin K antibody produced in mouse has been used in western blotting and enzyme linked immunosorbent assay (ELISA).

生化/生理作用

Cathepsin is involved in various functions such as intracellular degradation of exogenous and endogenous proteins, activation of enzyme precursors, and tumor invasion and metastasis. Among all the types, cathepsins K (Ctsk) is highly expressed in osteoclasts that plays a crucial role in bone remodeling and resorption. Cathepsin K protein was found in primary breast carcinoma and prostate cancer where it performs in the invasive process of prostate cancers, thyroid carcinoma, lung adenocarcinoma and breast carcinoma. Its deficency causes pycnodysostosis, a rare bone dysplasia with an osteopetrotic-like phenotype.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD02097890
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	CL124-1H6, monoclonal
形式	buffered aqueous solution
分子量	antigen ~27 kDa
species reactivity	human
浓度	~2 mg/mL
technique(s)	indirect ELISA: suitable western blot: 10-20 μg/mL using total cell extracts of HCT-116 cells
同位素/亚型	IgG2b
UniProt登记号	P43235
运输	dry ice
储存温度	?20℃
Gene Information	human ... CTSK(1513)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)