Creatinine Deiminase microbial

半岛bd体育手机客户端 说明

应用

Creatinine Deiminase microbial has been used:

• to immobilize aminosilylated glass beads based biosensor for ammonia/ammonium and creatinine detection in urine
• in creating creatinine-sensing membrane for biophysical studies
• to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples

Creatinine deiminase has been used in a study to assess the application of a creatinine-sensitive biosensor for hemodialysis control. Creatinine deiminase has also been used in a study to investigate the bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples.

包装

250 units in poly bottle

生化/生理作用

Creatinine deiminase catalyzes the hydrolysis of creatinine to methylhydantoine and ammonia.

物理属性

Isoelectric point : 4.4
Michaelis constant : 3.5 x 10￣³M (Creatinine)
Structure : 6 subunits per mol of enzyme
Inhibitors : Ag⁺,Hg⁺⁺, o-phenanthroline,monoiodoacetate
Optimum pH : 8.5 – 9.5
Optimum temperature : 65 – 75℃
pH Stability : pH 7.0 – 11.0 (30℃, 20hr)
Thermal stability : Below 65℃ (pH 7.5, 1hr)

单位定义

One unit will hydrolyze 1.0 μmole of creatinine to N-methylhydantoin and NH₃per min at pH 7.5 at 37 ℃ in a coupled system with L -glutamic dehydrogenase.

外形

Lyophilized powder containing mannitol as stabilizer

基本信息

酶学委员会(EC)编号	3.5.4.21 ( BRENDA | IUBMB )
EC 号	253-449-1
MDL编号	MFCD00130878
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	200
specific activity	≥25 units/mg protein
分子量	~260 kDa
组成	Protein, ≥15% biuret
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)