Cholesterol Oxidase from microorganisms

半岛bd体育手机客户端 说明

应用

Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that determined that cholesterol depletion impairs coupling between channel opening and vesicle release by allowing voltage-gated calcium channels to move further from release sites.
Cholesterol oxidase has been used in a study to improve treatment of oxysterol-mediated cytotoxicity. Cholesterol oxidase has also been used in a study that evaluated the effects of cholesterol depletion. This study reported that cholesterol depletion impairs the coupling between channel opening and vesicle release. Additionally, cholesterol oxidase is used to determine serum cholesterol levels. The enzyme also finds application in the microanalysis of steroids in food samples. It has also been used for distinguishing 3-ketosteroids from 3β-hydroxysteroids. Transgenic plants expressing cholesterol oxidase have been investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.

生化/生理作用

Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. The pH optimum of the enzyme is 7.0 to 7.5 and temperature optimum is 50 ℃. The pH stability is 5.7-7.8. Hg²⁺, Ag⁺, and ionic detergents inhibit the enzyme activity. Molecular mass of the enzyme is 62 kDa. Pathogenic bacteria require CHOD to infect a host′s macrophage.

单位定义

One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 ℃. Note: 4-cholesten-3-one may undergo isomerization.

制备说明

CHOD is soluble in cold 50 mM potassium phosphate buffer, pH 7.0. Prepare solutions immediately before use.

基本信息

酶学委员会(EC)编号	1.1.3.6 ( BRENDA | IUBMB )
EC 号	232-842-1
MDL编号	MFCD00130783
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
形式	aqueous solution
specific activity	≥30 units/mg protein (biuret)
分子量	62 kDa
溶解性	50 mM potassium phosphate buffer, pH 7.0: soluble (Cold)
运输	dry ice
储存温度	?70℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)