Anti-Cytokeratin, pan antibody, Mouse monoclonal antibody produced in mouse

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一般描述

Monoclonal Anti-Pan Cytokeratin (mouse IgG1 isotype) is derived from the PCK-26 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a cytokeratin preparation from human epidermis. Cytokeratins are a group of at least 29 different proteins. They are characteristic of epithelial and trichocytic cells. Cytokeratin peptide 1 (68 kDa) is expressed together with cytokeratin 10 in the suprabasal cell layers or the differentiation compartment of the epidermis. Its expression increases with epidermal maturation and is modified post translationally in terminally differentiated keratinocytes of the stratum corneum. Cytokeratin peptide 5 (58 kDa) is the primary type II keratin in stratified epithelia, while cytokeratin type 8 (52 kDa) is a major type II keratin in simple epithelia. Cytokeratin 6 (56 kDa) is a "hyperproliferation" cytokeratin expressed in tissues with natural or pathological high turnover. Monoclonal antibodies to cytokeratins are specific markers of epithelial cell differentiation and have been widely used as tools in tumor identification and classification.

特异性

该抗体可识别位于II型细胞角蛋白1、5、6和8上的表位。PCK-26是一种广谱抗体，可与多种正常、反应性和赘生性上皮组织发生特异性反应。该抗体可与简单、成角和非成角的鳞状上皮和假复层上皮反应。

免疫原

来源于人表皮的细胞角蛋白。

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)
Monoclonal Anti-Cytokeratin, pan antibody produced in mouse has been used in:

• immunoblotting
• immunohistochemistry
• Immunocytochemistry
• dot blotting

生化/生理作用

Cytokeratin facilitates the typing of normal, metaplastic, and neoplastic cells and may aid in the discrimination of carcinomas and non-epithelial tumors such as sarcomas, lymphomas, and neural tumors. It is also useful in detecting micrometastases in lymph nodes and other tissues, and for determining the origin of poorly differentiated tumors.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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