Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse

半岛bd体育手机客户端 说明

一般描述

Calpain-1 catalytic subunit is a protein encoded by the CAPN1 gene in humans. Calpains are calcium dependent proteases constituting a family of proteins. They share a homologous cysteine-protease domain and an E-F hand Ca²⁺-binding domain. The calpain system consists of two ubiquitous forms of calpain (m-calpain and μ-calpain), a tissue specific calpain (n-calpain) and a calpain inhibitory protein (calpastatin).

特异性

Epitope mapping studies indicate the epitope is between amino acids 465-520 (domain III) of human μ-calpain. The antibody reacts specifically with μ-calpain. It does not cross-react with m-calpain, n-calpain, calmodulin or calpastatin. It is not recommended for immunoprecipitation. By immunoblotting, reactivity is observed with human platelets and erythrocytes, bovine platelets, heart and skeletal muscle and with rat myoblasts, kidney, liver and spleen. By immunofluorescence on pig LLC-PK1 cells, diffuse cytoplasmic staining is observed.

免疫原

μ-calpain from bovine skeletal muscle.

应用

Monoclonal Anti-μ-Calpain (Domain III) antibody produced in mouse is suitable for indirect immunofluorescence at a dilution of 1:100 and western blotting at a dilution of 1:2,000.

生化/生理作用

Calpain is involved in calmodulin-independent pathway for the activation of calcineurin. Endogenous calpain I forms active calcineurin in the human heart by proteolysis of calcineurin A, which may lead to pathogenesis of myocardial disease. μ-calpain, on overexpression, may have relationship with intractable epilepsy as well as the clinicopathological characteristics. The activity of this protein is elevated in skeletal muscle from gastric cancer patients.

外形

Solution in phosphate buffered saline and contains 0.05% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD01322347
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	ascites fluid
antibody product type	primary antibodies
克隆	9A4H8D3, monoclonal
形式	buffered aqueous solution
分子量	antigen 80 kDa
species reactivity	rat, pig, human, bovine
technique(s)	indirect immunofluorescence: 1:100 western blot: 1:2,000
同位素/亚型	IgG1
UniProt登记号	P07384
运输	dry ice
储存温度	?20℃
Gene Information	human ... CAPN1(823) rat ... Capn1(29153)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	nwg
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)