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位置: 首页> 品牌> Sigma-Aldrich> Anti-E-Cadherin Antibody, clone 67A4, Azide &Ascites Free

Anti-E-Cadherin Antibody, clone 67A4, Azide &Ascites Free

品牌
Sigma-Aldrich
货号
MAB3199Z-C
规格纯度
clone 67A4, 0.5 mg/mL, from mouse
价格
5426.21 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-E-Cadherin Antibody, clone 67A4, Azide &Ascites Free
Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorulin, CD324
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Cadherins are a family of transmembrane glycoproteins involved in Ca2+-dependent cell to cell interactions. The members of the family are differentially expressed in various tissues and function in the maintenance of tissue integrity, morphogenesis, and migration. Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin), neural cadherin (N-cadherin), placental cadherin (P-cadherin) and retinal cadherin (R-cadherin). Type II include kidney cadherin (K-cadherin) and osteoblast cadherin (OB-cadherin). One of the best characterized cadherins is E-cadherin (epithelial cadherin or cadherin-1). It consists of calcium binding extracellular domains responsible for interactions with other E-cadherin molecules on neighboring cells and interaction with integrin alphaEbeta7. The cytoplasmic domain of E-cadherin is linked to the actin cytoskeleton through cytoplasmic catenin proteins, thus establishing a complex localized to adherens junctions. In carcinomas E-cadherin is frequently downregulated, which is consistent with its function of an invasion suppressor in normal epithelia.

免疫原

Whole cells corresponding to human E-Cadherin.

应用

Flow Cytometry Analysis: 2 ?g from a representative lot detected E-Cadherin in 1X10E6 A431 cells.

Flow Cytometry Analysis: A representative lot from an independent laboratory detected E-Cadherin in bone marrow cells (Burhing, H. J., et al. (1996). Leukemia. 10(1):106-116.)

Inhibition Analysis: A representative lot from an independent laboratory inhibited E-Cadherin-mediated adhesion of Langerhans to keratinocytes (Caberg, J. H., et al. (2008). Carcinogenesis. 29(7):1441-1447.).

Inhibition Analysis: A representative lot from an independent laboratory inhibits human embryoid body agglomeration (Dang, S. M., et al. (2004). Stem Cells. 22: 275-282.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).

Western Blotting Analysis: A representative lot from an independent laboratory detected E-Cadherin in human thymus tissue lysate (Kutlesa, S., et al. (2002). J Cell Sci. 115(Pt. 23):4505-4515.).
Research Category
Cell Structure
This Anti-E-Cadherin Antibody, clone 67A4, Azide & Ascites Free is validated for use in western blotting, flow cytometry, inhibition & immunofluorescence for the detection of E-Cadherin.
Research Sub Category
ECM Proteins

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: 0.25 ?g/mL of this antibody detected E-Cadherin in 10 ?g of A431 cell lysate.

目标描述

~97 kDa observed

联系

Replaces: 04-1103

外形

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified antibody
antibody product type primary antibodies
克隆 67A4, monoclonal
species reactivity human
浓度 0.5 mg/mL
technique(s) flow cytometry: suitable
immunofluorescence: suitable
inhibition assay: suitable
western blot: suitable
同位素/亚型 IgG1κ
NCBI登记号 NP_004351
UniProt登记号 P12830
运输 wet ice

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
闪点(F) Not applicable
闪点(C) Not applicable

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