Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free)

半岛bd体育手机客户端 说明

一般描述

Cytokeratin 18, also known as Keratin, type I cytoskeletal 18, Cell proliferation-inducing gene 46 protein, CK-18, Keratin-18, or K18, and encoded by the gene KRT18/CYK18/PIG46, is involved in the uptake of thrombin-anti-thrombin complexes by hepatic cells. When the protein is phosphorylated it plays a role in filament reorganization. Cytokeratin-18 together keratin-8 and IL-6 are involved in skin barrier protection too, and cytokeratin-18 is often complexed with Cytokeratin 8. Cytokeratin-18 is expressed in colon, placenta, liver epithelia and many other tissues. Cytokeratin 18 in epithelial cells is cleaved by caspases during apoptosis to produce apoptotic specific CK-18 fragment that is the basis of many apoptosis detection assays.

特异性

The antibody reacts with human cytokeratin 18 and is used for staining research samples of a wide variety of simple epithelia and simple glandular epithelia (e. g. intestinal, respiratory and urinary epithelia) and transitional epithelia (e. g. bladder), but stratified squamous epithelia are not stained (e. g. esophagus, epidermis).

免疫原

Cytoskeletal preparation of HeLa cells expressing human Cytokeratin 18.

应用

Research Category
Cell Structure
This Anti-Cytokeratin 18 Antibody, clone CK2 (Ascites Free) is validated for use in IHC, western blotting & immunofluorescence for the detection of Cytokeratin 18.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected Cytokeratin 18 in human placenta tissue.

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in bladder tissue (Moll, R., et al. (1988). Am. J. Pathol. 132(1): 123-44.).

Immunohistochemistry Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in ductal carcinoma of the breast, basal cell epithelioma, squamous cell carcinoma of the tongue, squamous cell carcinoma of epiglottis, and squamous cell carcinoma of the esophagus (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Western Blotting Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 HeLa cell lysate (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).

Immunofluorescence Analysis: A representative lot from an independent laboratory detected Cyokeratin 18 in human epithelia tissue, uterus (cervic glands), submucosal glands of the oesophagus, and placental tissue (Debus, E., et al. (1984). Am. J. Pathol. 114(4): 121-30.).
Research Sub Category
Cytokeratins

质量

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected Cytokeratin 18 in human colorectal cancer tissue.

目标描述

~48 kDa calculated

联系

Replaces: 04-586

外形

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	CK2, monoclonal
species reactivity	human
浓度	1 mg/mL
technique(s)	immunofluorescence: suitable immunohistochemistry: suitable western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_000215
UniProt登记号	P05783
运输	wet ice
Gene Information	human ... KRT18(3875)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable