Anti-CREB Antibody, clone LB9, Ascites Free

半岛bd体育手机客户端 说明

一般描述

Cyclic AMP-responsive element-binding protein 1 (UniProt P16220; also known as cAMP-responsive element-binding protein 1, CREB-1) is encoded by the CREB1 gene (Gene ID 1385) in human. CREB is a transcription factor that binds the cAMP response elements (CRE) and regulates the transcription of the downstream genes. CREB activity is regulation via phosphorylation on by various kinases, including PKA and Ca2+/calmodulin-dependent protein kinases. Genes known to be regulated by CREB include c-fos, BDNF, tyrosine hydroxylase, neuropeptides (such as somatostatin, enkephalin, VGF, and corticotropin-releasing hormone), as well as the circadian clock genes PER1 and PER2. CREB is critical for memory formation across a wide range of species.

特异性

Clone LB9 reactivity towarad spliced isoform CREB-B and htCREB (isoform 3) has not been determined.

应用

Anti-CREB Antibody, clone LB9, Ascites Free is an antibody against CREB for use in Western Blotting, Immunohistochemistry (Paraffin).
Western Blotting Analysis: 1.0 ?g/mL of this antibody from a representative lot detected CREB in 10 ?g of NIH/3T3 and PC12 cell lysate.
Immunohistochemistry Analysis: An 1:50-250 dilution of this antibody from a representative lot detected CREB in human liver, colon, and tonsil tissue sections.
Western Blotting Analysis: Representative lots detected CREB in primary mouse astrocytes and mouse dorsal hippocampal tissue lysates (Orr, A.G., et al. (2015). Nat. Neurosci. 18(3):423-434; Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).
Western Blotting Analysis: Representative lots detected CREB in primary human fibroblast and SH-SY5Y human neuroblastoma cell lysates (Maj, M., et al. (2011). Mitochondrion. 11(2):264-272; Fukuda, Y., et al. (2010). Mol. Cell Neurosci. 45(3):226-233).
Immunohistochemistry Analysis: A representative lot immunostained total CREB in dorsal hippocampal tissue sections from paraformaldehyde-perfused mice (Yiu, A.P., et al. (2011). Neuropsychopharmacology. 36(11):2169-2186).

质量

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 1.0 ?g/mL of this antibody detected CREB in 10 ?g of HeLa cell lysate.

目标描述

~37-43 kDa observed. 36.69 kDa (isoform CREB-A) calculated.

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	LB9, monoclonal
species reactivity	mouse, rat, human
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_604391
UniProt登记号	P16220
运输	wet ice
Gene Information	human ... CREB1(1385)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable