Anti-Metabotropic Glutamate Receptor 5 Antibody, pain

半岛bd体育手机客户端 说明

一般描述

Metabotropic glutamate receptor 5 or mGluR5 (UniProt Q3UVX5; also known as G protein-coupled receptor GRM5, G protein coupled receptor family C, group 1, member E) is encoded by the Grm5 (also known as Gprc1e, Mglur5, AI850523, 6430542K11Rik) gene in murine species (Gene ID 108071). The metabotropic glutamate receptors belong to a family of G protein-coupled receptors that have been divided into 3 groups based on their sequence homology, putative signal transduction mechanisms, and pharmacologic properties. mGluR1 and mGluR5 constitute Group I metabotropic glutamate receptors. mGluR5 is found on postsynaptic terminals of neurons and microglia. mGluR5 signals via Gq/G11 coupling to activate phospholipase C, resulting in calcium mobilization and activation of protein kinase C (PKC). In addition, mGluR5 is also reported to mediate signaling involving Src-family protein tyrosine kinase in a G protein-independent manner.

特异性

This antibody will not detect Isoform 2.

免疫原

KLH-conjugated linear peptide corresponding to the cytoplasmic domain of mouse Metabotropic Glutamate Receptor 5.

应用

Western Blotting Analysis: 0.1-0.5 μg/mL using ECL on rat brain microsomal preparation. Reacts with a band of ~132 kDa.
Immunohistochemistry: 0.1-0.2 μg/mL on rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Metabotropic Glutamate Receptor 5 in mouse hindbrain and rat colon tissue.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Anti-Metabotropic Glutamate Receptor 5 Antibody, pain is an antibody against Metabotropic Glutamate Receptor 5 for use in IH & WB.

质量

Evaluated by Western Blotting in microsomal preparation of rat brain tissue lysate.

Western Blotting Analysis: 2.0 ?g/mL of this antibody detected Metabotropic Glutamate Receptor 5 in 10 ?g of microsomal preparation of rat brain tissue lysate.

目标描述

~150 kDa observed. Uncharacterized band(s) may appear in some lysates.

联系

Replaces: AB1596; 06-451

外形

Purified rabbit polyclonal antibody in buffer containing PBS with 0.05% sodium azide.
ImmunoAffinity Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Brain tissue

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, rat
species reactivity (predicted by homology)	human (based on 100% sequence homology), bovine (based on 100% sequence homology), horse (based on 100% sequence homology), feline (based on 100% sequence homology), primate (based on 100% sequence homology)
manufacturer/tradename	Chemicon?
technique(s)	immunohistochemistry: suitable western blot: suitable
NCBI登记号	NP_001137306
UniProt登记号	Q3UVX5
运输	wet ice
Gene Information	human ... GRM5(2915)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable