EphB2/Fc Chimera from mouse

半岛bd体育手机客户端 说明

一般描述

EphB2 (Epherin receptor B2) is a receptor tyrosine kinase (RTK), which belongs to a 13 member family of ephrin receptors. This family is divided into two classes A and B. EphB2 belongs to EphB group, which contains five members. The ligands to this group of receptors are named ephrin-B1-B3. It is a bidirectional functioning membrane protein.

应用

EphB2/Fc Chimera from mouse has been used for the investigation of the role of ephrinB-EphB signaling in the induction and maintenance of hyperalgesia, hyperexcitability of neurons of DRG (dorsal root ganglion) and hyperexcitability and elevated synaptic plasticity of neurons of DH (dorsal horn) regions of brain.

生化/生理作用

EphB2 (Epherin receptor B2) is involved in the development and plasticity of glutamatergic synapses, and their interaction with NMDA receptor. It can also regulate the processing of acute inflammatory pain, and mechanical allodynia as a result of nerve crush. Activation of this receptor results in recruitment and activation of Src-protein kinases. These, in turn, phosphorylate and activate NMDA receptor. It regulates the function of platelets and the development of thrombus. It is also responsible for the retraction of clots, and modulates independent platelet function in a contact-independent manner. EphB2 signaling is also involved in the modulation of axonal sprouting, which is induced by lesions.
Member of the Eph receptor tyrosine kinase family shown to bind ephrin-B1, ephrin-B2, and ephrin-B3; involved in pattern formation and morphogenesis, and in arterial/venous demarcation of the developing vasculature.

其他说明

Extracellular domain of mouse EphB2 (amino acids 1-548) fused to the C-terminal 6X histidine-tagged Fc region of human IgG1 via a polypeptide linker.

外形

Lyophilized from a 0.2 μm filtered solution in 20 mM Tris, pH 8.0.

分析说明

The biological activity is measured by its ability to bind recombinant mouse ephrin-B2/Fc in an ELISA assay.

基本信息

MDL编号	MFCD01867526
NACRES	NA.32

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
重组	expressed in NSO cells
测定	>90% (SDS-PAGE)
形式	lyophilized powder
效能	0.04-2.5 ng/mL
分子量	monomer calculated mol wt 85.3 kDa 100-110 kDa by SDS-PAGE (reducing)
包装	pkg of 200 μg
储存条件	avoid repeated freeze/thaw cycles
technique(s)	ligand binding assay: suitable
杂质	endotoxin, tested
UniProt登记号	P54763
储存温度	?20℃
Gene Information	mouse ... Ephb2(13844)

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)