Goat Anti-Human IgG Antibody, biotin conjugate

半岛bd体育手机客户端 说明

一般描述

IgG is a monomeric immunoglobulin, consists of two heavy chains (gamma chains) and two light chains. Each molecule has two antigen binding sites. IgG is the most abundant immunoglobulin. It is approximately equally distributed in blood and in interstitial fluid, constituting 75% of serum immunoglobulins in humans. This is the only isotype that can pass through the human placenta, providing protection to the fetus in its first weeks of life before its own immune system has developed. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%).

特异性

The antibody reacts with the heavy chains of human IgG and with the light chains common to most human immunoglobulins.

免疫原

Prepared from purified human IgG.

应用

Research Category
Secondary & Control Antibodies
EIA: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Western blots: 1:20,000-1:400,000 using enzyme-streptavidin conjugate.
Immunohistochemistry: 1:500-1:5,000 using enzyme-streptavidin conjugate.
Flow cytometry: 1:200-1:1,000.
Fluorescence Immunohisto/cytochemistry: 1:200-1:1,1000.

Optimal working dilutions must be determined by the end user.
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Human IgG Antibody, biotin conjugate is validated for use in ELISA, WB for the detection of Human IgG.

外形

Purified by immunoaffinity chromatography using antigen coupled to agarose beads. Lyophilized. Buffer is 0.01 M Sodium Phosphate, 0.25 M NaCl, pH 7.6 with 15 mg/mL BSA, and 0.05% sodium azide.
ImmunoAffinity Purified

储存及稳定性

Maintain lyophilized product at 2°-8°C for up to 12 months from date of receipt. After reconstitution the product is stable for six weeks at 2°-8°C as an undiluted liquid. Reconstitute vial with 2 mL of distilled water. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of antibody (and buffer salts) will decrease to one-half of the original after the addition of glycerol and final working dilutions must be adjusted accordingly. Avoid repeated freeze thaw cycles.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.46

半岛bd体育手机客户端 性质

质量水平	100
生物来源	goat
偶联物	biotin conjugate
抗体形式	affinity purified immunoglobulin
antibody product type	secondary antibodies
克隆	polyclonal
species reactivity	human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable western blot: suitable
运输	wet ice

安全信息

象形图
警示用语：	Warning
危险声明	H312 + H332 - H412
预防措施声明	P261 - P271 - P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312
危险分类	Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Chronic 3
储存分类代码	11 - Combustible Solids
WGK	WGK 3