半岛bd体育手机客户端 说明
一般描述
The Monoclonal Anti-HA Biotin Conjugate antibody recognizes the HA tag sequence on the HA tagged fusion proteins when expressed at the N or C terminal to the fusion protein. This antibody is derived from the HA-7 hybridoma, which is produced from the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to the amino acid residues 98-106 (YPYDVPDYA) of human Influenza virus hemagglutinin (HA) known as HA-tag, conjugated to KLH. Ascites fluid collected from the HA-7 hybridoma is then conjugated to biotin.
特异性
该抗体可识别HA-标签蛋白的天然和还原变性形式，并且可与大肠杆菌或哺乳动物表达的N-末端或C-末端HA-标签融合蛋白发生反应。
免疫原
与人流感病毒血凝素(HA)片段相对应的合成肽，称为HA-标签，与KLH结合
应用
Antibody suitable for immunoblotting.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.
基本信息
| MDL编号 | MFCD00803873 |
| NACRES | NA.56 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 生物来源 | mouse |
| 偶联物 | biotin conjugate |
| 抗体形式 | purified from hybridoma cell culture |
| antibody product type | primary antibodies |
| 克隆 | HA-7, monoclonal |
| 形式 | buffered aqueous solution |
| 浓度 | ~1 mg/mL |
| technique(s) | western blot (chemiluminescent): 0.25-0.50 μg/mL using HA tagged fusion proteins in transiently transfected mammalian cell extracts. |
| 运输 | dry ice |
| 储存温度 | ?20℃ 2-8℃ |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
