Anti-Amyloid Antibody, β 1-40

半岛bd体育手机客户端 说明

一般描述

The cerebral and vascular plaques associated with Alzheimer′s disease (AD) are mainly composed of amyloid beta peptides (Ab). Ab is derived from cleavage of the amyloid precursor protein (APP) and varies in length from 39 to 43 amino acids. Ab [1-40], Ab [1-42], and Ab [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The cleavage takes place by gamma-secretase during the last APP processing step. Ab [1-40], [1-42] and [1-43] peptides are major constituents of the plaques and tangles that occur in AD. Ab antibodies and peptides have been developed as tools for elucidating the biology of AD.

特异性

Recognizes beta-amyloid 1-40. One of the most important and initial steps which causes loss of memory and cognition in Alzheimer′s Disease (AD) involves proteolytic cleavage of amyloid precursor protein (APP, chromosome 21) releasing short 40, 42 & 43 amino acid peptides (beta amyloid 1-40, 1-42 and 1-43). Polymerization of beta-amyloid and subsequent neuronal deposit (amyloid) leads to the degeneration of neurons involved in memory and cognition. The immunogen peptide shows homology with beta-amyloid 1-28 and beta-amyloid 12-28. No cross reactivity is observed with CGRP.

免疫原

A 7 amino acid peptide sequence from the C terminus of human beta-amyloid 1-40.

应用

Research Category
Neuroscience
Western blot: 1-10 μg/mL (Chemiluminescence technique)

Immunohistochemistry: 10-50 μg/mL using formalin or paraformaldehyde fixed Alzheimer′s brain tissue.

ELISA: 1:10,000-1:100,000 (50-100 ng immunogen peptide/well)

Optimal working dilutions must be determined by the end user.
Research Sub Category
Neurodegenerative Diseases
This Anti-Amyloid Antibody, β 1-40 is validated for use in ELISA, IH, WB for the detection of Amyloid.

外形

ImmunoAffinity Purified
Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA, no preservative.

储存及稳定性

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity purified immunoglobulin
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	mouse, human
manufacturer/tradename	Chemicon?
technique(s)	ELISA: suitable immunohistochemistry: suitable western blot: suitable
NCBI登记号	NM_000484.2 NM_201413.1 NM_201414.1
UniProt登记号	P05067
运输	dry ice
Gene Information	human ... APP(351)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 2
闪点(F)	Not applicable
闪点(C)	Not applicable