TSKgel^?Octadecyl-4PW HPLC Column

半岛bd体育手机客户端 说明

一般描述

Highly cross-linked polymethacrylate base material of TSKgel Octadecyl-4PW provides excellent stability in high pH buffer systems and can withstand rigorous cleaning with either acid or base. The 500? pore allows for anaylsis of proteins up to 200kDa while the particle size offerings allow for analytical, semi-preparative and preparative scale separations.
Polymer-based particles for reversed-phase (RP) HPLC generally have two benefits over silica-based particles: They are more tolerant of high and low pH operating conditions and they do not have the silanol interactions that cause peak tailing of some basic analytes. Also, some silicas have an intrinsic metal content that may cause chelation problems with sensitive biomolecules, leading to poor recovery. Polymeric TSKgel columns come in C18 and phenyl functional groups and a range of particle porosities. Fast kinetics and quantitative protein recovery at submicrogram loading are characteristic of nonporous resin-based TSKgel Octadecyl-NPR columns.

• Octadecyl-2PW particles have 125? pores
• Octadecyl-4PW particles have 500? pores
• Phenyl-5PW RP particles have 1000? pores
• Octadecyl-NPR particles are nonporous

外形

Shipped in 60% methanol - 40% water.

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法律信息

TSKgel is a registered trademark of Tosoh Corporation

基本信息

eCl@ss

32110501

半岛bd体育手机客户端 性质

物料	stainless steel hardware
半岛bd体育手机客户端 线	TSKgel?
manufacturer/tradename	Tosoh 18755
标记范围	1 meq/mL ligand density (estimate)
参数	no limitation salt concentration 0-100% organic solvent 0.22 mL/min flow rate 100 bar max. pressure 5-50 ℃ temperature
technique(s)	HPLC: suitable
长度 × 内径	15 cm × 2 mm
基质	polymer particle platform
基质活性基团	C18 (octadecyl) phase
粒径	7 μm
孔径	500 ?
操作pH	2 - 12
分离技术	reversed phase