半岛bd体育手机客户端 说明
一般描述
MANF, also known as ARMET, was initially identified as a protein containing an arginine-rich region that was highly mutated in a variety of tumors. More recently it was identified as a mesencephalic astrocyte-derived neurotrophic factor with selectivity for dopaminergic neurons, similar to glial cell line-derived neurotrophic factor (GDNF) and CDNF. In rat brain slices, MANF enhanced nigral gamma-aminobutyric acid release. Like GDNF and CDNF, MANF has selective neuroprotective activity for dopaminergic neurons suggesting that it may be indicated for the treatment of Parkinson’s disease. Expression of MANF has also been shown to be induced during ER stress, suggesting that it may play a role in protein quality control during ER stress.
特异性
This antibody does not cross-react with CDNF.
免疫原
KLH-conjugated linear peptide corresponding to human MANF.
应用
Detect the MANF protein using this Anti-MANF validated for use in WB & IHC.
Immunohistochemistry Analysis: A 1:2,000 dilution from a representative lot detected MANF in human midbrain and rat brain tissues.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
质量
Evaluated by Western Blot in PC12 cell lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected MANF in 15 ?g/mL of PC12 cell lysate.
目标描述
~18 kDa observed. The calculated molecular weight is 21 kDa, however MANF has been shown as an ~18 kDa band in western blots. (Mizobuchi, N., et al. (2007). Cell Structure and Function. 32:41-50.). Uncharacterized bands may appear at ~25 kDa and ~100 kDa in some lysates.
外形
Purified rabbit polyclonal in buffer containing PBS with 0.02% sodium azide.
Antigen Affinity Purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Control
PC12 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.41 |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 纯化方式 | affinity chromatography |
| species reactivity | human, mouse, rat |
| technique(s) | immunohistochemistry: suitable western blot: suitable |
| UniProt登记号 | P55145 |
| 运输 | wet ice |
| Gene Information | human ... MANF(7873) |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 2 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
