TBK1, active, GST tagged human

半岛bd体育手机客户端 说明

一般描述

TBK1 (TANK-binding kinase 1) gene is located on human chromosome 12q14. It is a serine/threonine protein kinase. The encoded protein has a kinase domain, a ubiquitin-like domain, an α-helical scaffold dimerization domain and a small adaptor-binding motif.

生化/生理作用

TBK1 (TANK-binding kinase 1) can phosphorylate numerous proteins, like optineurin, P62 (sequestosome 1) and interferon regulatory factor 3 (IRF3). It participates in various signaling pathways, namely, autophagy, antiviral and antibacterial innate immunity responses, oncogenic transformation and expression of inflammatory mediators. Mutations in this gene are linked with sporadic amyotrophic lateral sclerosis (SALS), familial ALS (FALS) and frontotemporal dementia. Defects in TBK1 suppresses autophagy, causing the accumulation of damaged mitochondria, thereby leading to neurodegeneration. Alteration in the copy number of TBK1 gene is associated with normal tension glaucoma. TBK1, also known as NAK or NFκB-activating kinase, is an upstream protein kinase that can phosphorylate and activate the IkB kinases. Activation of IkB kinases allows the phosphorylation of IkB protein which is then degraded via the ubiquitination pathway. This mechanism allows the activation of the NFκB transcriptional complex. TBK1 is a specific upstream regulator of IkB kinases and can also interact and the IkB protein TANK. TBK1 is a component of the virus-activated kinase that phosphorylate IRF3 and IRF7 allowing their dimerization and translocation to the nucleus, where they induce transcription of interferon.

外形

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

制备说明

after opening, aliquot into smaller quantities and store at -70 ℃. Avoid repeating handling and multiple freeze/thaw cycles

法律信息

PRECISIO is a registered trademark of sigma-aldrich Co. LLC

基本信息

NACRES

NA.32

半岛bd体育手机客户端 性质

重组	expressed in baculovirus infectedSf9 cells
半岛bd体育手机客户端 线	PRECISIO?Kinase
测定	≥70% (SDS-PAGE)
形式	buffered aqueous glycerol solution
specific activity	259-351 nmol/min·mg
分子量	~105 kDa
NCBI登记号	BC034950
运输	dry ice
储存温度	?70℃
Gene Information	human ... TBK1(29110)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1