LuminoCt^?SYBR^?Green qPCR ReadyMix^TM

半岛bd体育手机客户端 说明

一般描述

LuminoCt SYBR Green qPCR ReadyMix is designed to deliver unsurpassed assay speeds without sacrificing accuracy, precision, or sensitivity. Extensive design and validation of the ReadyMix chemistry has virtually eliminated the need for optimization when LuminoCt is used in conjunction with properly designed primers. Inclusion of JumpStart^?Taq antibody in the ReadyMix eliminates polymerase activity at ambient temperatures without causing the performance issues associated with chemically modified hot-start Taq. This allows for very rapid activation of the Taq and delivers unparalleled assay sensitivity, while allowing for benchtop reaction setup.

• Kit is designed to perform SYBR Green-based qPCR assays on commonly available real-time instrument platforms
• ReadyMix requires the addition of reference dye (provided in kit) when being used on real-time instruments that require ROX passive reference dye for normalization of qPCR assays
• Kit is not compatible with qPCR instruments that utilize glass capillary tubes

应用

LuminoCt^?SYBR^?Green qPCR ReadyMix^?has been used for fast qPCR and quantitative RT-PCR amplifications.

特点和优势

• Assay results in as little as 25 minutes
• Unsurpassed accuracy, precision, and sensitivity
• Virtually eliminates the need to optimize assay parameters

包装

Default reaction volume is 50 μL

100RXN is packaged as 1 X 2.5 mL
500RXN is packaged as 1 X 12.5 mL
2000RXN is packaged as 1 X 50 mL

其他说明

• At a minimum, use software to design primers for assays and ensure that qPCR amplicons are <200 bp
• Sigma recommends designing primers and probes with Beacon Designer software
• To order primers and probes from Sigma, visit: www.sigma.com/oligos

法律信息

Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of sigma-aldrich Co. LLC
LuminoCt is a registered trademark of sigma-aldrich Co. LLC
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
SabreLite is a registered trademark of Pelican Products, Inc.

基本信息

NACRES

NA.55

半岛bd体育手机客户端 性质

质量水平	200
形式	liquid
用途	sufficient for 100 reactions sufficient for 2000 reactions sufficient for 500 reactions
特点	dNTPs included hotstart
technique(s)	qPCR: suitable
颜色	colorless
input	purified DNA
application(s)	agriculture
相容性	for use with ABI 5700 for use with ABI 7000 for use with ABI 7300 for use with ABI 7500 Fast for use with ABI 7500 for use with ABI 7700 for use with ABI 7900 Fast for use with ABI 7900 HT for use with ABI 7900 for use with ABI StepOne for use with ABI StepOnePlus for use with ABI ViiA 7 for use with Bio-Rad CFX384 for use with Bio-Rad CFX96 for use with Bio-Rad MJ Chromo4 for use with Bio-Rad MJ Opticon 2 for use with Bio-Rad MJ Opticon Cepheid SmartCycler for use with Bio-Rad MJ Opticon for use with Bio-Rad MiniOpticon for use with Eppendorf?Mastercycler ep realplex2 s for use with Eppendorf?Mastercycler ep realplex for use with Illumina Eco qPCR for use with Qiagen Corbett Rotor-Gene 3000 for use with Qiagen Corbett Rotor-Gene 6000 for use with Qiagen Corbett Rotor-Gene Q for use with Roche LightCycler 480 for use with Strategene Mx3000P for use with Strategene Mx3005P for use with Strategene Mx4000
detection method	SYBR?Green
运输	wet ice
储存温度	?20℃

组份列表

组份可单独销售

货号	组份
	LuminoCt SYBR Green qPCR ReadyMix 2 X
R4526	100X ROX internal reference dye

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1