KiCqStart^?SYBR^?Green qPCR ReadyMix^TM

半岛bd体育手机客户端 说明

一般描述

KiCqStart SYBR Green qPCR ReadyMix is a 2X concentrated, ready-to-use reaction cocktail that contains all components, except primers and template, for real-time quantitative PCR (qPCR) This unique combination of proprietary buffer, stabilizers, and Hot-Start Taq DNA polymerase delivers maximum PCR efficiency, sensitivity, specificity and robust fluorescent signal using fast, or conventional, cycling protocols with SYBR Green qPCR.

Highly specific amplification is crucial to successful qPCR with SYBR Green I dye technology because this dye binds to and detects any dsDNA generated during amplification. Hot-Start Taq DNA polymerase is antibody mediated to be inactive prior to the initial PCR denaturation step. The optimized formulation includes SYBR Green I dye, an antibody-mediated Hot-Start Taq DNA polymerase, dNPTs, MgCl₂and proprietary buffers and stabilizers.

应用

KiCqStart^?SYBR^?Green qPCR ReadyMix^?has been used:

• in the amplification and quantification of cDNA reverse transcribed from RNA extracted from mice brain samples in a 2-step RT-qPCR assay
• to analyze DNA purified by ChIP technique
• to perform gene expression analysis

PCR applications:

• Gene expression
• DNA quantitation
• CHiP

特点和优势

• Assay results in as little as 33 minutes
• Highly efficient and sensitive real-time PCR results
• Little/no optimization required

组分

2X reaction buffer containing optimized concentrations of MgCl2, dNTPs, (dATP, dCTP, dGTP, dTTP), KiCqStart Taq DNA Polymerase, SYBR Green dye, ROX Reference Dye (for 580-585 nm excitation), and stabilizers.

packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume

其他说明

Storage Conditions:
KiCqStart SYBR Green qPCR ReadyMix is stable for 1 year when stored in a constant temperature freezer at -20℃, protected from light. For convenience, it may be stored unfrozen at +2 to +8℃ for up to 6 months. After thawing, mix thoroughly before using. Repeated freezing and thawing of the product is not recommended. However, the product demonstrated no loss of performance after 20 freeze-thaw cycles or 2 months at +20℃.

法律信息

6538 is a trademark of American Type Culture Collection
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of Qiagen Beverly Inc.
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of sigma-aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
StepOne is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
StepOnePlus is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries

基本信息

NACRES

NA.55

半岛bd体育手机客户端 性质

形式	liquid
用途	sufficient for 1250 reactions sufficient for 250 reactions sufficient for 5000 reactions
特点	dNTPs included hotstart
储存条件	protect from light
technique(s)	qPCR: suitable
颜色	colorless
input	purified DNA
相容性	for use with ABI 5700 for use with ABI 7000 for use with ABI 7300 for use with ABI 7700 for use with ABI 7900 HT Fast for use with ABI 7900 HT for use with ABI 7900 for use with ABI StepOne for use with ABI StepOnePlus
detection method	SYBR?Green
运输	dry ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable