半岛bd体育手机客户端 说明
一般描述
Inhibitor Resistant Genotyping PCR Readymix is a 2X concentrated ready-to-use master mix for PCR amplification of DNA templates that overcomes inhibitors often present in crude samples extracted from environmental specimens, plant tissues, or animal tissues. It is a versatile and robust PCR reagent that provides highly specific and efficient amplification for genotyping sequence variants including allelic discrimination of single nucleotide polymorphisms (SNPs) using a variety of fluorogenic probe chemistries, including TaqMan?hydrolysis probes. Inhibitor Resistant Genotyping PCR Readymix contains all required reaction components, except primers, probes, and DNA template. The light blue color of the inert tracer dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. It does not interfere with reporter dye fluorescence or affect the stability of the product.
A key component of Inhibitor Resistant Genotyping PCR ReadyMix is an ultra-pure, highly processive thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This proprietary polymerase mix is highly resistant to PCR inhibitors and provides an extremely stringent automatic hot-start allowing reaction assembly, and temporary storage, at room temperature prior to PCR amplification.
应用
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart?One-Step Probe RT-qPCR ReadyMix?does not contain an internal reference dye.
Inhibitor Resistant Genotyping PCR ReadyMix?has been used as a component of the reaction mixture for detection of leucoanthocyanidin reductase(PaLAR3) alleles in a genotyping assay and multiplex PCR for amplification of microsatellite.
特点和优势
组分
2X reaction buffer containing optimized concentrations of MgCl2, dNTPs (dATP, dCTP, dGTP, dTTP), hot-start DNA polymerase, insert blue qPCR dye, and stabilizers.
packaging:
250 reactions* = 2 X 1.25 mL tubes
1250 reactions* = 10 X 1.25 mL tubes
5000 reactions* = 1 X 50 mL tube
*number of reactions based on a 20uL volume
其他说明
Compatible instruments include:
法律信息
Bio-Rad is a registered trademark of Bio-Rad Laboratories, Inc.
CFX384 is a trademark of Bio-Rad Laboratories, Inc.
CFX96 is a trademark of Bio-Rad Laboratories, Inc.
Chromo4 is a trademark of Bio-Rad Laboratories, Inc.
Eppendorf is a registered trademark of Eppendorf AG
KiCqStart is a registered trademark of Qiagen Beverly Inc.
LightCycler is a registered trademark of Roche
Mastercycler is a registered trademark of Eppendorf AG
MiniOpticon is a trademark of Bio-Rad Laboratories, Inc.
Opticon is a trademark of Bio-Rad Laboratories, Inc.
ReadyMix is a trademark of sigma-aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
SmartCycler is a registered trademark of Cepheid, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
基本信息
| NACRES | NA.55 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 形式 | liquid |
| 用途 | sufficient for 1250 reactions sufficient for 250 reactions sufficient for 5000 reactions |
| 特点 | Difficult Templates/Specialty Enzymes PCR dNTPs included hotstart |
| 储存条件 | protect from light |
| technique(s) | qPCR: suitable |
| 颜色 | light blue |
| input | crude DNA |
| 相容性 | Bio-Rad CFX384 Bio-Rad CFX96 Bio-Rad MJ Chromo4 Bio-Rad MJ Opticon 2 Bio-Rad MJ Opticon Bio-Rad MiniOpticon Cepheid SmartCycler Eppendorf?Mastercycler ep realplex Eppendorf?Mastercycler ep realplex2 S Illumina Eco qPCR Qiagen Corbett Rotor-Gene 3000 Qiagen Corbett Rotor-Gene 6000 Qiagen Corbett Rotor-Gene Q Roche LightCycler 480 |
| detection method | probe-based |
| 运输 | dry ice |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 10 - Combustible liquids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
