半岛bd体育手机客户端 说明
一般描述
The 53-6.7 antibody reacts with the 32-34 kDa alpha subunit of mouse CD8, known as CD8a or CD8 alpha. CD8a can form a homodimer (CD8 alpha-alpha), but is more commonly expressed as a heterodimer with a second chain known as CD8b or CD8 beta. CD8 acts as a co-receptor in antigen recognition and subsequent T cell activation that is initiated upon binding of the T cell receptor (TCR) to antigen-bearing MHC Class I molecules. The cytoplasmic domains of CD8 provide binding sites for the tyrosine kinase lck, facilitating intracellular signaling events that lead to T cell activation, development, and cytotoxic effector functions. CD8+ cytotoxic T cells (CTLs) play an important role in inducing cell death of tumor cells, as well as cells infected by virus, bacteria or parasites.The 53-6.7 antibody is widely used as a phenotypic marker for mouse CD8a expression on cytotoxic T cells, thymocytes, as well as on certain cell types that do not also express the TCR, including some NK cells and lymphoid dendritic cells.
免疫原
Immunogen information for this antibody is proprietary information.
应用
Research Sub Category
Immunoglobulins & Immunology
This rat monoclonal Anti-CD8a Antibody (Mouse), FITC, clone 53-6.7, Cat. No. MABF1542 is validated for use in Flow Cytometry, for the detection of CD8a.
Research Category
Inflammation & Immunology
质量
This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.
Flow Cytometry Analysis: 0.5 μg from a representative lot detected CD8a in one million C57Bl/6 splenocytes.
外形
Purified Rat monoclonal containing 10 mM NaH2PO4, 150 mM NaCl, 0.09% NaN3, 0.1% gelatin, pH7.2.
Purified by size-exclusion chromatography
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
Note: It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
其他说明
Concentration: Please refer to lot specific datasheet.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| eCl@ss | 32160702 |
| NACRES | NA.44 |
半岛bd体育手机客户端 性质
| 质量水平 | 100 |
| 生物来源 | rat |
| 偶联物 | FITC conjugate |
| 抗体形式 | purified antibody |
| antibody product type | primary antibodies |
| 克隆 | 53-6.7, monoclonal |
| species reactivity | mouse |
| 包装 | antibody small pack of 25 μg |
| technique(s) | flow cytometry: suitable |
| 同位素/亚型 | IgG2aκ |
| UniProt登记号 | P01731 |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | nwg |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
