Human Fibroblast-Like Synoviocytes-Osteoarthritis: HFLS-OA, adult

半岛bd体育手机客户端 说明

一般描述

HFLS-RA provide an excellent cellular model for studying synoviocyte physiology in relation to development and treatment of rheumatoid arthritis.

HFLS-RA have been used in numerous research studies to:

• Study signaling pathways implicated in the development of joint inflammation and rheumatoid arthritis (Abe, 2006; Akasaka, 2005; Bai, 2012; Fan, 2013; Furuhashi, 2008; Hashizume, 2008; Matsuyama, 2010; Tsuji, 1999; Yamaguch, 2012a,b; Yano, 2001)
• Evaluate possible contribution of EBV to nonresolving rheumatoid arthritis inflammation through inducing IL-6 production by synoviocytes (Chiu, 2013)
• Evaluate the anti-inflammatory and antirheumatic activity of various compounds, such as celastrol (Venkatesha, 2011; Xu, 2013), triptolide (Kong, 2013; Lin, 2013), arsenic trioxide (Mei, 2011), Wen Luo Yin (Liu, 2013), bucillamine (Tsuji, 2007), therapeutic lyposomes (Harigai, 2007; Ichihara, 2011; Matsumoto, 2013; Negishi, 2011), anti-IL-6R and anti-CD319 antibodies (Suzuki, 2010; Woo, 2013), as well as inhibitors of NF-κB (Baima, 2010; Keith, 2005), methionine aminopeptidase-2 (Bernier, 2004), Tpl2 (Hall, 2007), aryl hydrocarbon receptor (Lahoti, 2013) and p38 MAP kinase (Wada, 2005); and additionally demonstrate the beneficial anti-inflammatory effects of low level light therapy (Yamaura, 2009)
• Investigate the causes of metalloproteinases induction in patients with Lyme disease-associated arthritis (Lin, 2001)
• Develop adenovirus-based siRNA delivery system (Arts, 2003)

Additionally, together with normal HFLS, HFLS-RA were used to:

• Identify causing agents (such as uric acid crystals or platelet microparticles) and study the immunopathological mechanisms and signal transduction pathways leading to joint inflammation in rheumatoid arthritis (Chen, 2011a; Hsu, 2012; Mathieu, 2008; Tsuji, 2012), and to demonstrate the role of estrogen signaling in increasing inflammation (Galal, 2008)
• Investigate anti-inflammatory properties of herbal compound Sinomenine suggested for rheumatoid arthritis treatment (Chen, 2011b)
• Study the effects of extracellular matrix composition on cell attachment and migration relevant to T-cell function in inflamed tissues (Evanko, 2012)

In parallel with HFLS isolated from joints of patients with osteoarthritis HFLS-RA were used in a study demonstrating the involvement of capsid proteins of parvorvirus B19 in activating synoviocyte migration and induction of the inflammatory response leading to acute symmetrical polyarthropathy (Lu, 2006).

Finally, all three types of HFLS (normal, RA and OA) were used to investigate the role of human endogenous retroviruses (HERVs) in development of rheumatoid arthritis, and suggest that activated expression of different forms of HERV contribute to development of rheumatoid arthritis symptoms by different mechanisms (Freimanis, 2010).

细胞系来源

Bone

应用

synoviocyte physiology in relation to development and treatment of rheumatoid arthritis, phenotype, proinflammatory mediator production, signaling pathways, interleukin production, evaluation of anti-inflammatory and antirheumatic compounds, erosive enzyme production (matrix metalloproteinases), cell proliferation and attraction

组分

Basal Medium containing 10% FBS & 10% DMSO

制备说明

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 5 doublings

传代培养常规

Please refer to the HFLS-OA Culture Protocol.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

生物来源	human synovial tissues (osteoarthritic)
质量水平	100
包装	pkg of 500,000 cells
生长模式	Adherent
核型	2n = 46
形态学	Fibroblast-like
technique(s)	cell culture | mammalian: suitable
相关疾病	arthritis; coagulation disorders
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable