Human Lung Fibroblasts: HLF, adult

半岛bd体育手机客户端 说明

一般描述

HLF provide an excellent model system to study many aspects of human pulmonary function and pathophysiology.

HLF have been utilized in a number of research publications, for example to:

• Demonstrate differential gene expression patterns between fetal and adult lung fibroblasts, in particular showing that fetal fibroblasts express higher level of TGF-β receptor interacting protein 1 (TRIP-1) and consequently are less able to contract collagen than adult lung fibroblasts, leading to better healing of acute injuries in very immature lungs compared to higher levels of fibrosis observed in older patients (Navarro, 2009), and additionally discover that TRIP-1 regulates lung fibroblast/myofibroblast trans-differentiation (Nyp, 2014)
• Show that angiotensin II promotes lung fibrosis by activating expression of IL-4, IL-7, and PDGF-D increasing the extracellular matrix formation, and that this process can be blocked by angiotensin receptor inhibitor candesartan (Okada, 2009)
• Determine that phosphorylation of C/EBPβ contributes to lung fibrosis (Buck, 2011)
• Demonstrate that increased tissue rigidity activates toll-like receptor 2 and NF-κB in lung fibroblasts and induces expression of cytokines, CXCL1-3, IL-8 and TNF-α (Zheng, 2011)
• Demonstrate that 2-ethoxyestradiol, a non-estrogenic estradiol metabolite analog, inhibits cell growth and retards the progression of pulmonary fibrosis and hypertension (Tofovic, 2008, 2009)
• Identify the hypoxia-induced ROS/RhoA–ROCK dependent actin reorganization as a mediator of myofibroblast transformation and collagen synthesis in lung fibrosis (Ni, 2013)

细胞系来源

Lungs

应用

pulmonary function, gene expression, healing and repair process, differentiation, extracellular matrix production, growth factor and cytokine activation, actin reorganization, synthesis of type II collagen, elastin, and proteoglycans, airway remodeling, pharmaceutical development

组分

Basal Medium containing 10% FBS & 10% DMSO

制备说明

• 1st passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 12 doublings

传代培养常规

Please refer to the HLF Culture Protocol.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

生物来源	human lung (normal parenchyma)
质量水平	100
包装	pkg of 500,000 cells
生长模式	Adherent
核型	2n = 46
形态学	fibroblast
technique(s)	cell culture | mammalian: suitable
相关疾病	asthma; tuberculosis; cardiovascular diseases; cancer
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable