Human Coronary Artery Smooth Muscle Cells: HCASMC, adult

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一般描述

Human Coronary Artery Smooth Muscle Cells (HCASMC) provide an excellent model system to study all aspects of cardiovascular function and disease, especially those related to mechanisms of hyperplasia and hypertrophy of intimal smooth muscle cells leading to vascular occlusion in atherosclerosis and stent restenosis.

HCASMC has been utilized in a number of research studies, for example, to:

• Study signaling pathways regulating smooth muscle differentiation (Zhou, 2010); and chronic inflammation of arterial wall that leads to artherosclerosis (Kiyan, 2014)
• Demonstrate that STAT-1 and STAT-3 regulate VEGF production in smooth muscle cells by having opposing effects on HIF-1α expression (Albasanz-Puig, 2012); study the mechanisms of hypoxia and reoxigenation injuries in by demonstrating increased production of ROS and inflammatory cytokines, and further showing that DHA is not beneficial in this type of injuries (Feng, 2012)
• Investigate the gene expression differences between smooth muscle cells from different arteries, underlying their differential response to injuries and proliferation stimuli (Lange, 2013)
• Suggest the hypermethylation of SOCS3 gene as the connection between TNF-α and IGF-1 released in response to mechanical injury during coronary intervention, and the induction of cytokines leading to intimal hyperplasia and restenosis (Dhar, 2013)
• Develop a novel VEGFR/MET-targeted inhibitor with improved antitumor efficacy and decreased toxicity (Fujita, 2013); and investigate novel therapies and drug combinations to achieve optimal target selectivity (Lehar, 2009; Wo-Wong, 2013)
• Develop elastic scaffolds for tissue engineering (Nivison-Smith, 2010, 2012) and novel treatment strategies to prevent stent restenosis by designing new materials (Crowder, 2012), or drug therapies to preferentially inhibit smooth muscle cell growth (O′Neill, 2009; Mociornita, 2013)

Characterization: positive for smooth muscle cell specific alpha-actin expression.

细胞系来源

Artery

应用

vascular research, supply of blood to heart muscle

组分

Basal Medium containing 10% FBS & 10% DMSO

制备说明

• 2nd passage, >500,000 cells in Basal Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

传代培养常规

Please refer to the HCASMC Culture Protocol.

基本信息

NACRES

NA.81

半岛bd体育手机客户端 性质

质量水平	100
生物来源	human coronary artery (normal, tunica intima and media)
包装	pkg of 500,000 cells
生长模式	Adherent
核型	2n = 46
形态学	smooth muscle
technique(s)	cell culture | mammalian: suitable
相关疾病	diabetes; stroke; cardiovascular diseases
运输	dry ice
储存温度	?196℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable