Acetyl-CoA carboxylase 2 human

半岛bd体育手机客户端 说明

应用

Acetyl-CoA carboxylase is responsible for synthesis of Malonyl-CoA which is an inhibitor of fatty acid oxidation in skeletal muscle mitochondria. The enzyme may be used to study the effect on production of malonyl-CoA as well as fatty acid oxidation during exercise. The enzyme also may be used for ACC regulation study in anti-obesity and anti-type 2 diabetes therapeutics.

生化/生理作用

Acetyl-CoA Carboxylase (ACC) regulates the metabolism of fatty acids. This enzyme catalzes the formation of Malonyl CoA through the irreversible carboxylation of acetyl CoA. There are two main isoforms of Acetyl-CoA carboxylase expressed in mammals, Acetyl-CoA carboxylase 1 (ACACA) and Acetyl-CoA carboxylase 2 (ACACB). ACACA has broad tissue distribution but is enriched in tissues critical for fatty acid sythesis such as adipose tissue. ACACB is enriched in tissues such as skeletal muscle and heart that are critical for fatty acid oxidation.

The Acetyl-CoA Carboxylase enzymes are activated by citrate, glutamate, and dicarboxylic acids and negatively regulated by long and short chain fatty acyl CoAs. Because of thier roles in fatty acid metabolism and oxidation, ACACA and ACACB are therapeutic targets for treating obesity and metabolic syndrome disorders.

物理属性

C-terminal HIS-tagged 277 kDa full length protein

单位定义

One unit will cause the carboxylation of 1 picomole of acetyl-CoA per minute at pH 7.4 at 30 deg C.

外形

Buffered aqueous solution containing Tris-HCl, pH 8.0, NaCl, glycerol, and DTT. May also contain one or more of the following: EDTA, KCl, imidazole, TWEEN-20.

制备说明

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70℃. Note: Enzyme is very sensitive to freeze/thaw cycles.

基本信息

酶学委员会(EC)编号	6.4.1.2 ( BRENDA | IUBMB )
NACRES	NA.26

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inSf9 cells
形式	solution
specific activity	≥25 units/μg protein
NCBI登记号	NM_001093 NP_001084
运输	dry ice
储存温度	?70℃
Gene Information	human ... ACACB(32)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable