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Anti-Actin, N-terminal antibody produced in rabbit

品牌
Sigma-Aldrich
货号
A2103
规格纯度
~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
价格
2038.93 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-Actin, N-terminal antibody produced in rabbit
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin.

Actin is one of the most conserved eukaryotic proteins, which is expressed in mammals and birds as at least six major different isoforms. Four of them represent the differentiation markers of muscle tissues and two are found practically in all non-muscle cells. Actin isoforms show >90% overall sequence homology, but only 50-60% homology in their 18 N-terminal residues. Actin is present in cells both as a globular monomer (G-actin) and as a polymer in filamentous actin (F-actin) that participates in the formation of a variety of stable and labile structures. The presence of actin in cell nuclei and cell membranes has been reported.

免疫原

synthetic actin N-terminal nonapeptide conjugated to KLH.

应用

Protein lysates generated from embryonic mouse limb tissue was analyzed by western blot using actin as the loading control. Actin was detected using rabbit anti-actin at 1:8000. Actin detected by a rabbit anti-actin antibody was used as a loading control for MCF7 cell lysates . Actin was detected at 42 kDa.
Anti-Actin, N-terminal antibody produced in rabbit has been used in

  • immunoblotting
  • quantitative chromatin immunoprecipitation (qChIP)


Rabbit anti-actin, N-terminal antibody can be used for western blotting assays. The antibody can also be used for immunocytochemistry at 1-2μg/mL using cultured chicken fibroblasts. Furthermore, the antibody is suitable for use in immunohistochemistry at a concentration of 2-4μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle.

生化/生理作用

Actin and myosin are constituents of many cell types and are involved in a myriad of cellular processes including maintenance of cell shape, locomotion, secretion, cytoplasmic streaming, phagocytosis, and cytokinesis. The N-terminal domain of actin appears to be a major antigenic region of the molecule. Different isoforms can coexist in the same cell but are differentially regulated, and in most cases cannot substitute for each other functionally.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.

其他说明

To view an Actin antibody selection guide, please visit www.sigmaaldrich.com/actin.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD00145889
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 42 kDa
species reactivity mouse, frog, rat, human, chicken
包装 antibody small pack of 25 μL
浓度 ~0.5 mg/mL
technique(s) immunocytochemistry: 1-2 μg/mL using cultured chicken fibroblasts
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2-4 μg/mL using sections of human appendix, mouse heart, and frog skeletal muscle
indirect immunofluorescence: suitable
western blot: 0.5-1 μg/mL using whole extract of the human epitheloid carcinoma HeLa cell line.
western blot: 2-4 ng/mL using whole extract of rat skeletal muscle
UniProt登记号 P63261
运输 wet ice
储存温度 ?20℃
Gene Information human ... ACTA1(58), ACTA2(59), ACTB(60), ACTC1(70), ACTG1(71), ACTG2(72)
mouse ... Acta1(11459), Acta2(11475), Actb(11461), Actc1(11464), Actg1(11465), Actg2(11468)
rat ... Acta1(29437), Acta2(81633), Actb(81822), Actc1(29275), Actg1(287876), Actg2(25365)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 2
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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